QIA76 Live/Dead Double Staining Kit

QIA76
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      Overview

      Replacement Information

      Key Specifications Table

      Detection Methods
      Fluorescence

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      QIA76-100TEST
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          Glass bottle 100 test
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          Description
          OverviewThis kit uses a cell-permeable green fluorescent Cyto-dye (Ex. max.: 488 nm; Em. max.: 518 nm) to stain live cells, and propidium iodide (Ex. max.: 488 nm; Em. max.: 615 nm) to stain dead cells. Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter which detects FITC and rhodamine. Viable cells will stain only with the Cyto-dye, fluorescing green, whereas the dead cells will stain with both Cyto-dye (green) and propidium iodide (red), resulting in a yellow fluorescence. Note: 1 T = 1 test.
          Optimal staining conditions may vary among different cell types and should be determined empirically by the investigator.
          Catalogue NumberQIA76
          Brand Family Calbiochem®
          Materials Required but Not Delivered Fluorescent microscope with a band-pass filter that detects FITC and rhodamine
          Microscope slides and cover slips
          References
          ReferencesLuther, E., and Kamenstsky, L.A. 1996. Cytometry 23, 272.
          Frey. T., et al. 1995. Cytometry 21, 265.
          Product Information
          Detection methodFluorescence
          Form100 Tests
          FormatFluorescence microscopy
          Kit containsStaining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol.
          Applications
          Biological Information
          Assay time0.5 h
          Sample TypeCell suspensions or adherent cells
          Species Reactivity
          • A Broad Range Of Species
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38-46

          Irritating to eyes, respiratory system and skin.
          May cause heritable genetic damage.
          S PhraseS: 26-36/37/39-45

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Intended useThe Calbiochem® Live/Dead Double Staining Kit can be used to distinguish between live and dead cells, which is essential for the study of growth control and cell death.
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage -20°C
          Storage ConditionsUpon arrival, store the entire contents of the kit at -20°C. Following initial use of the Staining Buffer, refrigerate (4°C) the remaining buffer.
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Kit containsStaining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol.
          Specifications

          Documentation

          Live/Dead Double Staining Kit SDS

          Title

          Safety Data Sheet (SDS) 

          Live/Dead Double Staining Kit Certificates of Analysis

          TitleLot Number
          QIA76

          References

          Reference overview
          Luther, E., and Kamenstsky, L.A. 1996. Cytometry 23, 272.
          Frey. T., et al. 1995. Cytometry 21, 265.

          Brochure

          Title
          Biologics 33.2
          User Protocol

          Revision15-March-2017 JSW
          Form100 Tests
          FormatFluorescence microscopy
          Detection methodFluorescence
          Speciesa broad range of species
          StorageUpon arrival, store the entire contents of the kit at -20°C. Following initial use of the Staining Buffer, refrigerate (4°C) the remaining buffer.
          Intended useThe Calbiochem® Live/Dead Double Staining Kit can be used to distinguish between live and dead cells, which is essential for the study of growth control and cell death.
          Principles of the assayThe Calbiochem® Live/Dead Double Staining Kit provides ready-to-use staining reagents to conveniently discriminate between live and dead cells. The kit utilizes Cyto-dye, a cell-permeable green flourescent dye (Ex/Em = 488/518 nm), to stain live cells. Dead cells can be easily stained by propidium iodide (PI), a non-permeable red flourescent dye (Ex/Em= 488/615) that can only enter the cell when there is membrane damage that results in permeabilization. Stained live and dead cells can be visualized by flourescence microscopy using a band-pass filter that detects FITC and rhodamine. Cyto-Dye has also been used to discriminate between apoptotic and non-apoptotic cells.
          Materials provided• Solution A (Kit Component No. JA1897): 1 vial, 50 µl, 1 mM Cyto-Dye
          • Solution B (Kit Component No. JA1898): 1 vial, 50 µl, 1 mg/ml Propidium Iodide (PI)
          • Staining Buffer (Kit Component No. JA1899): 1 vial, 50 ml
          Materials Required but not provided Fluorescent microscope with a band-pass filter that detects FITC and rhodamine
          Microscope slides and cover slips
          Reagent preparation• Preparation of Staining Solution: Prior to use, prepare enough Staining Solution for the number of cell samples being tested. For example, to prepare 1 ml Staining Solution, mix 1 µl Solution A and 1 µl Solution B in 1 ml Staining Buffer. Use a ratio of 100 µl Staining Solution per 1 x 105 cells.
          Detailed protocol1. Collect cells by centrifugation at 500 x g for 5 min and discard the supernatant. For analyzing adherent cells, grow the cells directly on a coverslip.
          2. Resuspend the cell pellet with Staining Solution. Use 100 µl for every 1 x 105 cells.
          3. Incubate for 15 min at 37°C. For analyzing adherent cells, stain the cells directly on the coverslip.
          4. Transfer an aliquot of the cell suspension to a glass slide and cover the cells with a glass coverslip. For analyzing adherent cells, invert the coverslip on a glass slide to visualize the cells.
          5. Analyze the cells immediately under a fluorescence microscope using a band-pass filter that detects flourescein and rhodamine. Live cells stain only the cell-permeable Cyto-Dye, flourescing green. Dead cells stain with both the cell-permeable Cyto-Dye and the non-permeable propidium iodide, fluorescing red; the overlay of green and red appears yellow.

          Note: The optimal staining conditions may vary among different cell types, so it is recommended that the optimal concentration of Solution A and B be determined for individual cell types.
          Registered TrademarksCalbiochem® is a registered trademark of EMD Millipore, Corp., Inc.
          InteractivePathways™ is a trademark of EMD Millipore Corp.