429700 | Leptin, Human, Recombinant, E. coli

429700
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      429700-1MG
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          Plastic ampoule 1 mg
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          Description
          OverviewRecombinant, human leptin expressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations of the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism, and body temperature. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis. Note: Following complete dissolution in 15 mM HCl, add 7.5 mM sterile NaOH and bring the pH to approximately 5.2.
          Catalogue Number429700
          Brand Family Calbiochem®
          SynonymsrhOB
          References
          ReferencesAnderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
          Ookuma, M., et al. 1998. Diabetes 47, 219.
          Campfield, L.A., et al. 1995. Science 269, 546.
          Halaas, J.L., et al. 1995. Science 269, 543.
          Pelleymounter, M.A., et al. 1995. Science 269, 540.
          Zhang, Y., et al. 1994. Nature 372, 425.
          Product Information
          CAS number177404-21-6
          FormLyophilized
          FormulationLyophilized from a sterile filtered solution in PBS.
          Applications
          Biological Information
          Biological activityED₅₀ = 0.4-2 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells
          Purity≥97% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤1.0 EU/µg leptin
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsTo reconstitute lyophilized leptin, add 15 mM sterile HCl (0.5 ml/1 mg vial or 2.5 ml/5 mg vial) to the vial. After the protein is completely dissolved, add 7.5 mM sterile NaOH (0.3 ml/1mg vial or 1.5 ml/5 mg vial) to bring the pH to ~5.2. Lyophilized samples are stable for at least six months at -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 4°C for one month or at -70°C for three months without detectable loss of activity. Avoid repeated freeze/thaw cycles of reconstituted solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          429700

          References

          Reference overview
          Anderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
          Ookuma, M., et al. 1998. Diabetes 47, 219.
          Campfield, L.A., et al. 1995. Science 269, 546.
          Halaas, J.L., et al. 1995. Science 269, 543.
          Pelleymounter, M.A., et al. 1995. Science 269, 540.
          Zhang, Y., et al. 1994. Nature 372, 425.

          Brochure

          Title
          Calbiochem Biologics 32.2
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision14-May-2008 RFH
          SynonymsrhOB
          DescriptionRecombinant, human leptin expressed in E. coli. Leptin was originally identified as a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding leptin have been isolated from human, simian, mouse and rat cells. Human leptin shares approximately 84% sequence identity with the mouse protein. Human leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid signal sequence that is cleaved to yield the 146 amino acid mature protein. The expression of leptin mRNA has been shown to be restricted to adipose tissue.

          A high-affinity receptor for leptin (OB-R) with homology to gp130 and the G-CSF receptor was subsequently cloned. The OB-R cytoplasmic domain transduces the leptin signal through the JAK-STAT pathway. Although OB-R mRNA was initially shown to be expressed predominantly in the choroid plexus and in the hypothalamus, more recent data also revealed the expression of this receptor in endothelial cells (Ecs). Furthermore, the angiogenic activity of leptin has been demonstrated both in vitro and in vivo, suggesting a physical mechanism whereby leptin-induced angiogenesis may facilitate increased energy expenditure.
          FormLyophilized
          FormulationLyophilized from a sterile filtered solution in PBS.
          CAS number177404-21-6
          Purity≥97% by SDS-PAGE
          ContaminantsEndotoxin: ≤1.0 EU/µg leptin
          Biological activityED₅₀ = 0.4-2 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsTo reconstitute lyophilized leptin, add 15 mM sterile HCl (0.5 ml/1 mg vial or 2.5 ml/5 mg vial) to the vial. After the protein is completely dissolved, add 7.5 mM sterile NaOH (0.3 ml/1mg vial or 1.5 ml/5 mg vial) to bring the pH to ~5.2. Lyophilized samples are stable for at least six months at -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 4°C for one month or at -70°C for three months without detectable loss of activity. Avoid repeated freeze/thaw cycles of reconstituted solutions.
          Toxicity Standard Handling
          Merck USA index14, 5443
          ReferencesAnderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
          Ookuma, M., et al. 1998. Diabetes 47, 219.
          Campfield, L.A., et al. 1995. Science 269, 546.
          Halaas, J.L., et al. 1995. Science 269, 543.
          Pelleymounter, M.A., et al. 1995. Science 269, 540.
          Zhang, Y., et al. 1994. Nature 372, 425.

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          Categories

          Life Science Research > Proteins and Enzymes > Other Proteins