Key Specifications Table
|Key Applications||Detection Methods|
|TFX, IF, ICC||Fluorescent|
|Description||LentiBrite™ GFP Control Lentiviral Biosensor|
|Overview||Read our application note in Nature Methods!
Learn more about the advantages of our LentiBrite Lentiviral Biosensors! Click Here
Biosensors can be used to detect the presence/absence of a particular protein as well as the subcellular location of that protein within the live state of a cell. Fluorescent tags are often desired as a means to visualize the protein of interest within a cell by either fluorescent microscopy or time-lapse video capture. Visualizing live cells without disruption allows researchers to observe cellular conditions in real time.
Lentiviral vector systems are a popular research tool used to introduce gene products into cells. Lentiviral transfection has advantages over non-viral methods such as chemical-based transfection including higher-efficiency transfection of dividing and non-dividing cells, long-term stable expression of the transgene, and low immunogenicity.
EMD Millipore is introducing LentiBrite™ Lentiviral Biosensors, a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.
EMD Millipore’s LentiBrite™ GFP Control lentiviral particles provide bright fluorescence to enable live-cell analysis in difficult-to-transfect cell types.
|Background Information||The use of genetically encoded fluorescent proteins has revolutionized cell biology by enabling real-time imaging of cells and subcellular structures. A diverse palette of fluorescent proteins is available, but these proteins vary in their suitability for live-cell imaging. EMD Millipore’s LentiBriteTM GFP Control Lentiviral Biosensor employs TagGFP2, the improved variant of the Aequorea macrodactyla GFP-like protein. TagGFP2 exhibits bright green fluorescence comparable to that of EGFP, with excitation/emission maxima at 483 and 506 nm, respectively. TagGFP2 is specially optimized for expression at 37°C and is therefore well-suited for use in mammalian cells. EMD Millipore’s LentiBrite™ GFP Control lentiviral particles provide bright fluorescence to enable live-cell analysis in difficult-to-transfect cell types.|
|Purification Method||PEG precipitation|
|Safety Information according to GHS|
|Product Usage Statements|
|Quality Assurance||Evaluated by transduction of HT-1080 cells and fluorescent imaging performed for assessment of transduction efficiency.|
|Material Size||1 vial (minimum of 3 x 10E8 IFU/mL)|
|LentiBrite GFP Control Lentiviral Biosensor - Q2068463||Q2068463|
|LentiBrite GFP Control -2515765||2515765|
|LentiBrite GFP Control -2556373||2556373|
|LentiBrite GFP Control -2788676||2788676|