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14-598 | Lambda Protein Phosphatase, GST, 250 µg

250 µg  
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      Replacement Information
      Catalogue Number14-598
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionLambda Protein Phosphatase, GST, 250 µg
      OverviewFull-length, recombinant Lambda Protein Phosphatase (l-PPase) with a N- terminal GST-tag, l-PPase is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine, tyrosine.

      Also included with the Lambda Protein Phosphatase, GST are a Dilution Buffer (#20-264) and a Reducing Buffer (#20-265).
      Product Information
      ApplicationFull-length, recombinant Lambda Protein Phosphatase (λ-PPase) with a N-terminal GST-tag. λ-PPase is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine, tyrosine. For use in Phosphatase Assays.
      Key Applications
      • Phosphatase Assay
      Application NotesDephosphorylation
      Biological Information
      Sourceexpressed in E. coli.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined.
      Gene Symbol
      • PPM1F
      • POPX2
      • CaMKPase
      • FEM-2
      • hFEM-2
      • Ca
      • KIAA0015
      Protein TargetLambda Protein Phosphatase
      Purification MethodGlutathione agarose affinity chromatography
      Target Sub-FamilySer/Thr/Tyr/His phosphatase
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P49593 # Dephosphorylates and concomitantly deactivates CaM- kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.
      COFACTOR: Binds 2 magnesium or manganese ions per subunit (By similarity).
      SIZE: 454 amino acids; 49831 Da
      SUBUNIT: Associates with F1Aalpha.
      SIMILARITY: SwissProt: P49593 ## Belongs to the PP2C family.
      Molecular Weight50kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceUsed to measure the hydrolysis of p-nitrophenyl phosphate (pNPP).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Lambda Protein Phosphatase, GST (recombinant protein expressed in E. coli) - 2033874A 2033874A
      Lambda Protein Phosphatase, GST (recombinant protein expressed in E. coli) - 2107404 2107404
      Lambda Protein Phosphatase, GST (recombinant protein expressed in E. coli) - 2329790 2329790
      Lambda Protein Phosphatase, GST (recombinant protein expressed in E. coli) - 2033874 2033874
      Lambda Protein Phosphatase, GST - 26929 26929
      Lambda Protein Phosphatase, GST - DAM1458083 DAM1458083
      Lambda Protein Phosphatase, GST - DAM1523956 DAM1523956
      Lambda Protein Phosphatase, GST - JBC1881356 JBC1881356


      Reference overviewPub Med ID
      Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase.
      Zhuo, S, et al.
      J. Biol. Chem., 268: 17754-61 (1993) 1993

      Show Abstract
      8394350 8394350
      Discovery of a protein phosphatase activity encoded in the genome of bacteriophage lambda. Probable identity with open reading frame 221.
      Cohen, P T and Cohen, P
      Biochem. J., 260: 931-4 (1989) 1989

      Show Abstract
      2548489 2548489