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3301 | LIGHT DIAGNOSTICS™ Coxsackievirus A9 Reagent, ~25 tests, included in kit #3350

1 mL  
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      Key Specifications Table

      Key ApplicationsFormatHostDetection Methods
      IF Purified M Fluorescent
      Catalogue Number3301
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionLIGHT DIAGNOSTICS™ Coxsackievirus A9 Reagent, ~25 tests, included in kit #3350
      OverviewLight Diagnostics type specific monoclonal antibody for Coxsackievirus A9 is intended for use in indirect fluorescence screening for the presumptive identification of Coxsackievirus A9 obtained in cell culture and not intended for testing directly on human specimens.

      Test Principle

      Light Diagnostics Coxsackievirus A9 Monoclonal Antibody (MAb Cox A9) can be used to identify a Coxsackie A9 viral isolate in cell culture using an indirect immunofluorescence assay (IFA). The monoclonal antibody provided will bind to the type specific Coxsackie A9 isolate present on the cell culture slide. Unbound monoclonal antibody is removed by rinsing with phosphate buffered saline (PBS). A secondary FITC (fluorescein isothiocyanate) labeled antibody is then added which will bind to the antigen-antibody complex. Unbound secondary antibody is removed by rinsing with PBS. FITC exhibits an apple green fluorescence when illuminated by ultraviolet light allowing visualization of the complex by microscopy. A positive result is indicated by specific cell fluorescence. Non-infected cells stain a dull red if Evans Blue counterstain is used in the FITC-labeled secondary antibody or used elsewhere in the procedure.

      Background and Clinical Significance:

      Enteroviruses are classified to be in the picornavirus family, pico [small] + RNA [ribonucleic acid] + virus. Picornaviruses are among the smallest and simplest ribonucleic acid containing viruses known [1]. The RNA for many enteroviruses have now been cloned and complete genomic sequences have been obtained. The RNA from all the sequenced enteroviruses are similar in length, about 7400 nucleotides, and have identical organization [1].

      The human alimentary tract is the predominant site of enterovirus replication and the viruses were first isolated from enteric specimens. These viruses are the causes of paralytic poliomyelitis, aseptic meningitis-encephalitis, myocarditis, pleurodynia, hand-foot-and-mouth disease, conjunctivitis, and numerous other syndromes associated with extra-intestinal target organs. There are 67 numbered types of enteroviruses in the enteroviruses family [1]: Polioviruses (3), Coxsackieviruses A (23), Coxsackieviruses B (6), Echoviruses (31), and other Enteroviruses (4)

      Enteroviruses, including Echoviruses and Coxsackieviruses, have been reported as the major etiologic agents of aseptic meningitis [2]. Clinical syndromes associated with infections by each type of enterovirus have also been reported [3]. Coxsackievirus A9 can cause aseptic meningitis, paralysis, exanthema, pneumonitis of infants, and hepatitis.

      Establishing an association between an enterovirus and a particular disease in a patient requires laboratory confirmation of infection, usually by either isolation of the virus, or documentation of a specific serologic response in a properly timed specimen. Detailed descriptions of principals and procedures for diagnosis of enterovirus infections have been published [4-7]. Cell culture techniques have made the accurate detection of enteroviruses possible [8-10]. The identification of the enterovirus isolates will help prevention, treatment and understanding of the infectious diseases, and even discovery of new virus isolates. The typing of enterovirus isolates is generally accomplished by neutralization with type specific pools of immune sera [11]. This method is time consuming (7 days or more) and expensive. As an alternative, typing of enteroviruses with type specific monoclonal antibody and/or group specific monoclonal antibody pool(s) by the Indirect Immunofluorescence Assay (IFA) is potentially more rapid and less expensive [12-18].
      Materials Required but Not Delivered· Acetone, reagent grade; stored in glass.

      · Distilled water.

      · Sodium hypochlorite solution, 0.05% (1:100 dilution of household bleach).

      · Tissue culture tubes or shell-vials with 12 mm coverslips containing monolayer of cell line appropriate for growth of Enteroviruses.

      · Tissue culture media (RPMI or Eagle's Minimum Essential Medium with fetal bovine serum and antibiotics, or equivalent).

      · Viral transport medium.

      · 0.lN NaOH.

      · 0.1N HCl.

      · Microscope slides, non-fluorescing.

      · No. 1 cover slips.

      · Anti-Mouse IgG/FITC Conjugate (Catalog No. 5008).

      · Normal Mouse Antibody to be used as negative control.

      · Phosphate Buffer Saline (PBS, 0.01 M pH 7.1-7.4 with 0.085% NaCl and 0.1% Azide), (Catalog No. 5087).

      · 0.05% Tween 20/0.1% Sodium Azide Solution (optional), (Catalog No. 5037).

      · Aspirator device with disposable Pasteur pipettes.

      · Centrifuge capable of 700-950 x g with biohazard buckets and adapters for shell-vials.

      · Fluorescence microscope with appropriate filter combination for FITC (excitation peak = 490 nm, emission peak = 520 nm) with 100x, 200x, 400x, magnification (dry objective).

      · Forceps.

      · Humid chamber.

      · Incubator, 37 ± 1°C.

      · Syringe filter, 0.45 micron.

      · Ultrasonic water bath.

      · Vortex mixer or sonicator.

      · Mounting Media (Catalog No. 5013).

      · Coxsackie A9 / A24 Control Slides (Catalog No. 5076).
      Product Information
      • Coxsackie A9 Virus Monoclonal Antibody - (Catalog No. 3301).
      • One dropper vial containing 1 mL, sufficient for 25 tests, ready to use, mouse IgG(2b) monoclonal antibody against the Coxsackie A9 virus, protein stabilizer, and 0.1 % sodium azide (preservative).
      Detection methodFluorescent
      Key Applications
      • Immunofluorescence
      Biological Information
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For in vitro Diagnostic Use
      • CE Mark
      Storage and Shipping Information
      Storage ConditionsWhen stored at 2-8°C, the monoclonal antibody is stable up to the expiration date printed on the label. Avoid multiple freeze and thaw.

      Warning and Precautions:

      · For in vitro diagnostic use.

      · The performance of Light Diagnostics Coxsackie A9 MAb has not been determined on direct specimens.

      · Sodium azide, present in the reagents, can form potentially explosive metal azides with lead and copper pipes. As a precaution, flush with large amount of water to prevent azide build-up.

      · Do not allow the slides to dry at any time during the staining procedure.

      · Slides prepared too early (<25% CPE) or too late (>95% CPE) can be difficult to read and can lead to false negatives.

      · Handle all specimens and materials coming in contact with them as potentially infectious materials. All samples should be handled at the Biosafety Level 2 as recommended for any potentially infectious material in the Center for Disease Control/National Institute of Health Manual, "Biosafety in Microbiological and Biomedical Laboratories," (1984). Decontaminate with 0.05% sodium hypochlorite.

      · Avoid contact with Evans Blue if present in any reagent as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.

      · Do not mouth pipette reagents.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      References | 14 Available | See All References

      Reference overviewPub Med ID
      Tyrosine residues at the carboxyl terminus of Vav1 play an important role in regulation of its biological activity.
      Galit Lazer,Liron Pe'er,Marganit Farago,Kazuya Machida,Bruce J Mayer,Shulamit Katzav
      The Journal of biological chemistry 285 2010

      Show Abstract Full Text Article
      20457609 20457609
      Evaluation of insecticidal activity of diterpenes and lignans from Aristolochia malmeana against Anticarsia gemmatalis.
      Gisele B Messiano,Leandro Vieira,Marcos B Machado,Lucia M X Lopes,Sergio A de Bortoli,Julio Zukerman-Schpector
      Journal of agricultural and food chemistry 56 2008

      Show Abstract
      18380460 18380460
      High histone acetylation and decreased polycomb repressive complex 2 member levels regulate gene specific transcriptional changes during early embryonic stem cell differentiation induced by retinoic acid.
      Elliot R Lee,Fern E Murdoch,Michael K Fritsch
      Stem cells (Dayton, Ohio) 25 2007

      Show Abstract
      17525233 17525233
      The role of histone acetylation in regulating early gene expression patterns during early embryonic stem cell differentiation.
      McCool, KW; Xu, X; Singer, DB; Murdoch, FE; Fritsch, MK
      The Journal of biological chemistry 282 6696-706 2007

      Show Abstract
      17204470 17204470
      Upregulation of acid-sensing ion channel ASIC1a in spinal dorsal horn neurons contributes to inflammatory pain hypersensitivity.
      Bo Duan,Long-Jun Wu,Yao-Qing Yu,Yu Ding,Liang Jing,Lin Xu,Jun Chen,Tian-Le Xu
      The Journal of neuroscience : the official journal of the Society for Neuroscience 27 2007

      Show Abstract
      17928456 17928456
      Activity of PXD101, a histone deacetylase inhibitor, in preclinical ovarian cancer studies.
      Xiaozhong Qian,William J LaRochelle,Gulshan Ara,Frank Wu,Kamille Dumong Petersen,Annemette Thougaard,Maxwell Sehested,Henri S Lichenstein,Michael Jeffers
      Molecular cancer therapeutics 5 2006

      Show Abstract
      16928830 16928830
      Synthesis of light-activated antisense oligodeoxynucleotide.
      XinJing Tang,Ivan J Dmochowski
      Nature protocols 1 2006

      Show Abstract
      17406566 17406566
      Nanostructured ordering of fluorescent markers and single proteins on substrates.
      Juergen Groll,Krystyna Albrecht,Peter Gasteier,Silke Riethmueller,Ulrich Ziener,Martin Moeller
      Chembiochem : a European journal of chemical biology 6 2005

      Show Abstract
      16175540 16175540
      Structural and functional properties of chicken lysozyme fused serine-rich heptapeptides at the C-terminus.
      Xiaohua Xu,Orie Kashima,Akira Saito,Hiroyuki Azakami,Akio Kato
      Bioscience, biotechnology, and biochemistry 68 2004

      Show Abstract
      15215591 15215591
      Proteasome-dependent degradation of cyclin D1 in 1-methyl-4-phenylpyridinium ion (MPP+)-induced cell cycle arrest.
      Jie Bai,Hajime Nakamura,Shugo Ueda,Yong-Won Kwon,Toru Tanaka,Sadayuki Ban,Junji Yodoi
      The Journal of biological chemistry 279 2004

      Show Abstract
      15247282 15247282

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      LIGHT DIAGNOSTICS™ A9 MONOCLONAL ANTIBODY, ~25 tests, included in kit #3350