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14-330 | JNK2α2/SAPK1a Protein, unactive, 50 µg

50 µg  
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      Replacement Information
      Catalogue Number14-330
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionJNK2α2/SAPK1a Protein, unactive, 50 µg
      OverviewN-terminal His6-tagged, recombinant, full-length, human JNK2α2/SAPK1a unactive
      Product Information
      ApplicationUnactive, N-terminal His6-tagged, recombinant, full-length, human JNK2α2/SAPK1a unactive, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
      Gene Symbol
      • MAPK9
      • JNK2B
      • JNK-55
      • JNK2
      • JNK2BETA
      • p54aSAPK
      • JNK2ALPHA
      • PRKM9
      • JNK2A
      Protein TargetJNK2α2/SAPK1a
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P45984 # JNK2 isoforms display different binding patterns: alpha- 1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.
      COFACTOR: Magnesium.
      SIZE: 424 amino acids; 48139 Da
      SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway.
      DOMAIN: SwissProt: P45984 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Autophosphorylated in vitro.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight49.2kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of JNK2α2/SAPK1a
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      JNK 2#225;2/SAPK1a, unactive - 1640776 1640776
      JNK 2#945;2/SAPK1a, unactive - 2052230-A 2052230-A


      Reference overviewPub Med ID
      Selective interaction of JNK protein kinase isoforms with transcription factors.
      Gupta, S, et al.
      EMBO J., 15: 2760-70 (1996) 1996

      Show Abstract
      8654373 8654373
      Transcription factor ATF2 regulation by the JNK signal transduction pathway.
      Gupta, S, et al.
      Science, 267: 389-93 (1995) 1995

      Show Abstract
      7824938 7824938

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