420108 | JNK Substrate, c-Jun 1-79 - Calbiochem

420108
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      420108-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecombinant c-Jun (1-79) activation domain fused to GST and expressed in E. coli. Highly specific substrate for c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK). Is not phosphorylated by p42 MAP kinase or p38. Suitable for qualitative and quantitative assays of JNK activity. Shown to undergo phosphorylation by JNK in the presence of 32P-ATP.
          Catalogue Number420108
          Brand Family Calbiochem®
          SynonymsSAPK Substrate
          References
          ReferencesDerijard, B., et al. 1994. Cell 76, 1025.
          Kyriakis, J.M., et al. 1994. Nature 369, 156.
          Hibi, M., et al. 1993. Genes Dev. 7, 2135.
          Product Information
          ATP CompetitiveN
          FormLiquid
          FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 2 mM EDTA, 1 mM DTT, 50% glycerol, pH 7.5.
          ReversibleN
          Applications
          Biological Information
          Biological activityShown to undergo phosphorylation by JNK in the presence of ³²P-ATP.
          Primary TargetHighly specific substrate for c-Jun N-terminal kinase/stress activated protein kinase (JNK/SAPK)
          Purity≥90% by SDS-PAGE
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          420108

          References

          Reference overview
          Derijard, B., et al. 1994. Cell 76, 1025.
          Kyriakis, J.M., et al. 1994. Nature 369, 156.
          Hibi, M., et al. 1993. Genes Dev. 7, 2135.

          Brochure

          Title
          MAP Kinases Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-September-2018 JSW
          SynonymsSAPK Substrate
          DescriptionGST fusion protein of c-jun (1-79) activation domain, expressed in E. coli. Highly specific substrate form c-jun N-terminal kinase/stress activated protein kinase (JNK/SAPK). Not phosphorylated by MAP kinase or p38. Suitable for qualitative and quantitative assays of JNK activity.
          FormLiquid
          FormulationIn 50 mM NaCl, 20 mM Tris-HCl, 2 mM EDTA, 1 mM DTT, 50% glycerol, pH 7.5.
          Purity≥90% by SDS-PAGE
          Biological activityShown to undergo phosphorylation by JNK in the presence of ³²P-ATP.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesDerijard, B., et al. 1994. Cell 76, 1025.
          Kyriakis, J.M., et al. 1994. Nature 369, 156.
          Hibi, M., et al. 1993. Genes Dev. 7, 2135.