420200 | JC-1 - CAS 47729-63-5 - Calbiochem

420200
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₅H₂₇Cl₄IN₄ 47729-63-5

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      420200-5MG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Plastic ampoule 5 mg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewA cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨm in cells, tissues, and isolated mitochondria. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of ΔΨm induced by uncouplers of mitochondrial respiration. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.
          Catalogue Number420200
          Brand Family Calbiochem®
          Synonyms5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide
          References
          ReferencesMathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
          Gravance, C.G., et al. 2000. Theriogenology 53, 1691.
          Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
          Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
          Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
          White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
          Ankacrona, M., et al. 1995. Neuron 15, 961.
          Reers, M., et al. 1995. Methods Enzymol. 260, 406.
          Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.
          Product Information
          CAS number47729-63-5
          ATP CompetitiveN
          FormRed-orange solid
          Hill FormulaC₂₅H₂₇Cl₄IN₄
          Chemical formulaC₂₅H₂₇Cl₄IN₄
          ReversibleN
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Primary TargetUsed as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria
          Purity≥95% by HPLC
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          420200

          References

          Reference overview
          Mathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
          Gravance, C.G., et al. 2000. Theriogenology 53, 1691.
          Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
          Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
          Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
          White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
          Ankacrona, M., et al. 1995. Neuron 15, 961.
          Reers, M., et al. 1995. Methods Enzymol. 260, 406.
          Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision28-July-2008 RFH
          Synonyms5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide
          DescriptionA cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨm in cells, tissues, and isolated mitochondria. It labels mitochondria with high membrane potential orange and mitochondria with low membrane potential green. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. Ideal for detection of collapse of ΔΨm induced by uncouplers of mitochondrial respiration. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.
          FormRed-orange solid
          Intert gas (Yes/No) Packaged under inert gas
          CAS number47729-63-5
          Chemical formulaC₂₅H₂₇Cl₄IN₄
          Structure formulaStructure formula
          Purity≥95% by HPLC
          SolubilityDMSO (5 mg/ml)
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.
          Toxicity Irritant
          ReferencesMathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
          Gravance, C.G., et al. 2000. Theriogenology 53, 1691.
          Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
          Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
          Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
          White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
          Ankacrona, M., et al. 1995. Neuron 15, 961.
          Reers, M., et al. 1995. Methods Enzymol. 260, 406.
          Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.