A part of MilliporeSigma

14-162 | Inhibitor 2, PP1 specific

100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service


      Contact Customer Service

      Click To Print This Page


      Replacement Information
      Catalogue Number14-162
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionInhibitor 2, PP1 specific
      Overviewexpressed in E. coli.
      Product Information
      ApplicationInhibits PP1 from many sources (human,
      rabbit, yeast). For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceHuman recombinant Inhibitor-2 derived from a full-length cDNA (please see Roach, et al., JBC, 260: 6314, 1985 and Helps, et al., FEBS Letters 340: 93-98, 1994).
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • PPP1CA
      • PP-1A
      • MGC15877
      • MGC1674
      • PPP1A
      Protein TargetPP-1A
      Purification MethodIon-exchange chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62136 # Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II.
      COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
      SIZE: 330 amino acids; 37512 Da
      SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A and PPP1R12B mediate binding to myosin. PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen. Interacts with PPP1R9A and PPP1R9B (By similarity). Interacts with PPP1R7.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity).
      SIMILARITY: SwissProt: P62136 ## Belongs to the PPP phosphatase family. PP-1 subfamily.
      Molecular Weight32kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by a protein phosphatase assay
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -70°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Inhibitor 2 (specific inhibitor of PP1) - 2187210 2187210
      Inhibitor 2 (specific inhibitor of PP1) - 2277839 2277839
      Inhibitor 2 - 16429 16429
      Inhibitor 2 - 17622 17622
      Inhibitor 2 - 18478 18478
      Inhibitor 2 - 2048885 2048885
      Inhibitor 2 - 26521 26521
      Inhibitor 2 - DAM1606769 DAM1606769
      Inhibitor 2 - DAM1791371 DAM1791371
      Inhibitor 2 - JBC1863285 JBC1863285


      Reference overviewPub Med ID
      Cloning of the complete coding region for human protein phosphatase inhibitor 2 using the two hybrid system and expression of inhibitor 2 in E. coli.
      Helps, N R, et al.
      FEBS Lett., 340: 93-8 (1994) 1994

      Show Abstract
      8119416 8119416
      Expression of the catalytic subunit of phosphorylase phosphatase (protein phosphatase-1) in Escherichia coli.
      Zhang, A J, et al.
      J. Biol. Chem., 267: 1484-90 (1992) 1992

      Show Abstract
      1730696 1730696
      Protein phosphatase inhibitor-1 and inhibitor-2 from rabbit skeletal muscle.
      Cohen, P, et al.
      Meth. Enzymol., 159: 427-37 (1988) 1988

      2842607 2842607
      Phosphoprotein phosphatase inhibitor-2. Identification as a species of molecular weight 31,000 in rabbit muscle, liver, and other tissues.
      Roach, P, et al.
      J. Biol. Chem., 260: 6314-7 (1985) 1985

      Show Abstract
      2987219 2987219
      The protein phosphatases involved in cellular regulation. 1. Classification and substrate specificities.
      Ingebritsen, T S and Cohen, P
      Eur. J. Biochem., 132: 255-61 (1983) 1983

      Show Abstract
      6301824 6301824