401002 | Iberiotoxin, Buthus tamulus, Recombinant, E. coli - CAS 129203-60-7 - Calbiochem

401002
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #
      129203-60-7

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      401002-10UG
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          Plastic ampoule 10 μg
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          Description
          OverviewRecombinant Buthus tamulus iberiotoxin expressed in E. coli. Acts as a selective, reversible blocker of high conductance Ca2+-activated K+ channels (IC50 = 250 pM).
          Catalogue Number401002
          Brand Family Calbiochem®
          SynonymsrlbTx, Pyr-FTDVDCSVSKECWSVCKDLFGVDRGKCMGKKCRCYQ
          References
          ReferencesVanelli, G., et al. 1994. J. Appl. Physiol. 76, 1098.
          Garcia, M.L., et al. 1991. J. Bioeng. Biomembr. 23, 615.
          Galvez, A., et al. 1990. J. Biol. Chem. 265, 11083.
          Product Information
          CAS number129203-60-7
          ATP CompetitiveN
          FormSalt-free lyophilized solid
          ReversibleY
          Applications
          Biological Information
          Biological activitypotently inhibits Ca2+-activated K+ channels expressed in Xenopus oocytes
          Primary TargetHigh conductance Ca2+-activated K+ channels
          Primary Target IC<sub>50</sub>250 pM against high conductance Ca2+-activated K+ channels
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableN
          Peptide SequencePyr-Phe-Thr-Asp-Val-Asp-Cys⁷-Ser-Val-Ser-Lys-Glu-Cys¹³-Trp-Ser-Val-Cys¹⁷-Lys-Asp-Leu-Phe-Gly-Val-Asp-Arg-Gly-Lys-Cys²⁸-Met-Gly-Lys-Lys-Cys³³-Arg-Cys³⁵-Tyr-Gln-OH (disulfide bonds: 7 →28; 13 → 35; 17 → 33)
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 20/21/22

          Harmful by inhalation, in contact with skin and if swallowed.
          S PhraseS: 36

          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 3 months at -20°C or up to 2 weeks at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          401002

          References

          Reference overview
          Vanelli, G., et al. 1994. J. Appl. Physiol. 76, 1098.
          Garcia, M.L., et al. 1991. J. Bioeng. Biomembr. 23, 615.
          Galvez, A., et al. 1990. J. Biol. Chem. 265, 11083.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision14-July-2008 RFH
          SynonymsrlbTx, Pyr-FTDVDCSVSKECWSVCKDLFGVDRGKCMGKKCRCYQ
          DescriptionRecombinant Buthus tamulus iberiotoxin expressed in E. coli. A single polypeptide chain of 37 amino acids that displays about 68% sequence homology with charybdotoxin (ChTx). Acts as a selective, reversible blocker of high conductance, Ca2+-activated K+ channels (PK,Ca). rIbTx does not block other types of voltage-dependent ion channels, especially other types of K+ channels that are sensitive to inhibition by ChTx. Shown to block PK,Ca in excised membrane patches from bovine aortic smooth muscle, acting exclusively at the outer face of the channel (IC50 = 250 pM). Causes a decrease in probability of channel opening and the channel open mean time. rIbTx appears to modulate ChTx binding by an allosteric mechanism.
          FormSalt-free lyophilized solid
          CAS number129203-60-7
          Peptide SequencePyr-Phe-Thr-Asp-Val-Asp-Cys⁷-Ser-Val-Ser-Lys-Glu-Cys¹³-Trp-Ser-Val-Cys¹⁷-Lys-Asp-Leu-Phe-Gly-Val-Asp-Arg-Gly-Lys-Cys²⁸-Met-Gly-Lys-Lys-Cys³³-Arg-Cys³⁵-Tyr-Gln-OH (disulfide bonds: 7 →28; 13 → 35; 17 → 33)
          Purity≥98% by HPLC
          Biological activitypotently inhibits Ca2+-activated K+ channels expressed in Xenopus oocytes
          SolubilityReconstitute in Tris-buffered saline (100 mM NaCl, 10 mM Tris-HCl, pH 7.5) containing 0.1% BSA.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 3 months at -20°C or up to 2 weeks at 4°C.
          Toxicity Harmful
          ReferencesVanelli, G., et al. 1994. J. Appl. Physiol. 76, 1098.
          Garcia, M.L., et al. 1991. J. Bioeng. Biomembr. 23, 615.
          Galvez, A., et al. 1990. J. Biol. Chem. 265, 11083.