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13-124 | IRS1

13-124
20 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number13-124
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionIRS1
      OverviewN-terminal 6His-tagged, recombinant rat IRS1, expressed in Sf9 cells.
      Alternate Names
      • Insulin Receptor Substrate 1
      • pp185
      References
      Product Information
      Presentation50mM Tris-HCl, 150mM NaCl, 250mM Imidazole, pH7.5, and 10% glycerol. Frozen liquid.
      Applications
      Key Applications
      • Western Blotting
      Biological Information
      PurityPurity >50%, determined by SDS-PAGE and Coomassie blue staining.
      SourceRat IRS1 expressed in Sf9 cells.
      Entrez Gene Number
      Gene Symbol
      • IRS1
      • IRS-1
      • HIRS-1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
      SIZE: 1242 amino acids; 131591 Da
      SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.
      PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
      SIMILARITY: SwissProt: P35568 ## Contains 1 IRS-type PTB domain. & Contains 1 PH domain.
      Molecular Weight165 kDa (including 6His-Tag)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by detecting IRS1 with anti-IRS1 (Cat. No. 06-248 or 06-526)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -70°C for up to 1 year from date of receipt.
      Packaging Information
      Material Size20 µg
      Transport Information
      Supplemental Information
      Specifications