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14-461-D | IKKα

10 μg  
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      Key Specifications Table

      Uni Prot Number
      Catalogue Number14-461-D
      Brand Family Upstate
      Trade Name
      • Upstate
      OverviewRecombinant human IKKα, also known as CHUK, IKK1, IKBKA, TCF16, and NFKBIKA, full-length expressed in a baculovirus-infected Sf9 insect cell expression system
      Product Information
      Presentation50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutatione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
      ApplicationRecombinant human IKKα, also known as CHUK, IKK1, IKBKA, TCF16, and NFKBIKA, full-length expressed in a baculovirus-infected Sf9 insect cell expression system, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
      Key Applications
      • Kinase Assay
      Biological Information
      Concentration0.1 mg/ml
      SourceSf9 insect cells
      Specific Activity2.5 pmol/min/µg
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
      Gene Symbol
      • CHUK
      • NFKBIKA
      • IKKA
      • IKK-A
      • IKBKA
      • IKK1
      • IKK-alpha
      • IkBKA
      • TCF16
      Protein TargetIKKα
      Target Sub-FamilyMisc
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O15111 # Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B- regulated promoters during inflammatory responses triggered by cytokines.
      SIZE: 745 amino acids; 84654 Da
      SUBUNIT: Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP (By similarity). Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. A weak interaction with TRAF2 cannot be excluded. Part of a complex composed of NCOA2, NCOA3, IKKB, IKBKG and CREBBP. Interacts with NALP2. May interact with MAVS/IPS1.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus.
      TISSUE SPECIFICITY: Widely expressed.
      PTM: Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
      SIMILARITY: SwissProt: O15111 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight114 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Kinase assay: 10 µl of varying amounts of IKKβ was mixed with 5 µl of 1 mg/ml IKKtide synthetic peptide substrate and 5 µl of distilled water. Reaction was initiated by adding 5 µl of 250 µM [33P]-ATP and incubating for 15 min at 30°C followed by spotting the reaction on phosphocellulose paper, fixing in 1% phosphoric acid, and assaying with a scintillation counter.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for > 6 month at –80°C from date of receipt.
      Packaging Information
      Material Size10 μg
      Transport Information
      Supplemental Information