534331 Hypochlorous Acid Fluorescent Probe, PIS

534331
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₁₅H₁₂N₄S

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      5.34331.0001
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          Glass bottle 10 mg
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          Description
          OverviewA cell-permeable imidazoline-2-thione based compound that acts as a direct, rapid, selective and sensitive fluorescence turn-on detection probe for hypochlorous acid (HOCl; detection limit of 71 nM). Efficiently reacts with hypochlorite to yield fluorescent imidazolium ions, with good water solubility and photostability. Generates negligible fluorescence emission with several other ROS/RNS (H2O2, TBHP, NO·, ROO·, ONOO and HO·). Shown to efficiently visualize and detect stimulants triggered endogenous HOCl generation in RAW 264.7 macrophages co-cultured with HeLa cells. Also enables to monitor HOCl levels by two-photon image in RAW 264.7 cells and in rat hippocampal slices (depth of >100 µm) in a dose-dependent manner.
          Catalogue Number534331
          Brand Family Calbiochem®
          Synonyms1,3-Dimethyl-1H-imidazo[4,5-b]phenazine-2(3H)-thione, Hypochlorite Fluorescent Probe, MPO Activity Probe
          DescriptionHypochlorous Acid Fluorescent Probe, PIS
          References
          ReferencesXu, Q., et al. 2015. Angew. Chem. Int. Ed. 54, 4890.
          Product Information
          FormBrown solid
          Hill FormulaC₁₅H₁₂N₄S
          Chemical formulaC₁₅H₁₂N₄S
          Applications
          Biological Information
          Primary Targethypochlorous acid
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableY
          Emission max.
          Excitation max.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Hypochlorous Acid Fluorescent Probe, PIS SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Xu, Q., et al. 2015. Angew. Chem. Int. Ed. 54, 4890.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-June-2016 JSW
          Synonyms1,3-Dimethyl-1H-imidazo[4,5-b]phenazine-2(3H)-thione, Hypochlorite Fluorescent Probe, MPO Activity Probe
          DescriptionA cell-permeable imidazoline-2-thione based compound that acts as a direct, rapid, selective and sensitive fluorescence turn-on detection probe for hypochlorous acid (HOCl; detection limit of 71 nM). Efficiently reacts with hypochlorite to yield fluorescent imidazolium ions, with good water solubility and photostability. Generates negligible fluorescence emission with several other ROS/RNS (H2O2, TBHP, NO•, ROO•, ONOO- and HO•. Shown to efficiently visualize and detect stimulants triggered endogenous HOCl generation in RAW 264.7 macrophages co-cultured with HeLa cells. Dose-dependently images HOCl levels by two-photon fluorescence microscopy in RAW 264.7 cells and in rat hippocampal slices (depth of >100 µm).
          FormBrown solid
          Intert gas (Yes/No) Packaged under inert gas
          Chemical formulaC₁₅H₁₂N₄S
          Purity≥98% by HPLC
          SolubilityDMSO (0.5 mg/ml) or DMF (0.5 mg/ml). Small yellow particles may be visible.
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesXu, Q., et al. 2015. Angew. Chem. Int. Ed. 54, 4890.