389561 Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.

389561
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      389561-100U
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          Glass bottle 100 u
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          Description
          OverviewNative hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.
          Catalogue Number389561
          Brand Family Calbiochem®
          References
          ReferencesBaker, J.R., et al. 2002. Biochem. J. 365, 317.
          Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.
          Product Information
          CAS number37326-33-3
          Activity≥ 100 U/ampule [1 U = 1 TRU (Turbidity Reducing Unit)]
          Unit of DefinitionOne TRU (Turbidity Reducing Unit = 1 U) is defined as the amount of enzyme that will hydrolyze 50% of hyaluronic acid, determined by a 50% reduction in turbidity, in 30 min at 60°C pH 6.0.
          EC number3.2.1.35
          FormWhite lyophilized powder
          Applications
          Biological Information
          Specific Activity≥2000 U/A280
          Physicochemical Information
          ContaminantsProtease: ≤ 0.05 units/ampule (pH 6.0)
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions prepared in water are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          389561

          References

          Reference overview
          Baker, J.R., et al. 2002. Biochem. J. 365, 317.
          Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-July-2009 JSW
          DescriptionNative hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.

          Please Note: The appearance of this product can vary from gray cottony fibers to balled up droplets with no change in quality.
          FormWhite lyophilized powder
          Recommended reaction conditionsAssay for Enzymatic Activity Reagent Preparation Prepare acetate buffer: 150 mM NaCl, 20 mM sodium acetate-acetic acid buffer, pH 6.0. Prepare hyaluronic acid solution: 50 mg purified potassium hyaluronate (containing approximately 38% glucuronic acid) is dissolved in 100 ml of the above mentioned sodium acetate buffer. Measure acidic bovine serum albumin with a spectrophotometer at 280 nm. Adjust the total absorbance of the albumin solution to 910. Dilute to 100 ml with 0.5 N acetate buffer, pH 4.3. Adjust the pH of the albumin solution to 3.1 using 4 N HCl. Boil in a water bath for 30 min at 100°C. Adjust to 100 ml with distilled H2O. This solution is stable for 3-4 weeks at 4°C. To use this solution, dilute with 5 volumes of acetate buffer from above. Activity Protocol For the assay, mix 0.5 ml of the enzyme solution (made with acetate buffer without NaCl) with 0.5 ml of the hyaluronic acid solution. Incubate for 30 min at 60°C. Add 4 ml of the acidic bovine serum albumin solution. Incubate for 10 min at 60°C. Measure the turbidity at 660 nm. Obtain a blank value by using deactivated enzyme (incubate at 100°C for 10 min). Correct the reading by subtracting the blank value. (blank value - measured value/blank value) x 2 x 2 x dilution = TRU/ml
          CAS number37326-33-3
          EC number3.2.1.35
          ContaminantsProtease: ≤ 0.05 units/ampule (pH 6.0)
          Specific activity≥2000 U/A280
          Activity≥ 100 U/ampule [1 U = 1 TRU (Turbidity Reducing Unit)]
          Unit definitionOne TRU (Turbidity Reducing Unit = 1 U) is defined as the amount of enzyme that will hydrolyze 50% of hyaluronic acid, determined by a 50% reduction in turbidity, in 30 min at 60°C pH 6.0.
          Solubility500 mM Acetate buffer, pH 6.0 (30 U/ml) or H₂O (30 U/ml)
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions prepared in water are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.
          Toxicity Standard Handling
          Merck USA index14, 4758
          ReferencesBaker, J.R., et al. 2002. Biochem. J. 365, 317.
          Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.