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FC010-5MG | Human Plasma Fibronectin Purified Protein

FC010-5MG
5 mg  5 x 1 mg
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      CULT
      Description
      Catalogue NumberFC010-5MG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHuman Plasma Fibronectin Purified Protein
      OverviewHuman fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.
      References
      Product Information
      PresentationSupplied in 5 individual vials. Liquid in 150 mM NaCl, 10 mM sodium phosphate, pH 7.5, sterile filtered, containing no preservatives.
      Applications
      Key Applications
      • Cell Culture
      Application NotesImmunochemical standard

      Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts

      Suggested Procedure for Coating Cell Cultureware

      1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.

      2.Reconstitute HFN at 1mg/mL and dilute to the desired concentration with physiological buffer.

      3.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

      4. Introduce the proper CO2 atmosphere, if required.

      5. Add the calculated amount of HFN to each culture vessel.

      6. Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.

      7. Remove residual balanced salt solution before proceeding with standard cell culture procedures
      Biological Information
      Concentration1 mg/mL
      PurityApproximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. HFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.
      SourceHuman donor plasma.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
      Gene Symbol
      • FN1
      • DKFZp686O13149
      • DKFZp686H0342
      • DKFZp686I1370
      • MSF
      • FN
      • CIG
      • DKFZp686F10164
      • LETS
      • FINC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
      SIZE: 2386 amino acids; 262607 Da
      SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
      TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
      PTM: Sulfated.
      SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.
      Packaging Information
      Material Size5 mg
      Material Package5 x 1 mg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2147051 2147051
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2382597 2382597
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2445845 2445845
      HUMAN PLASMA FIBRONECTIN -2567113 2567113
      HUMAN PLASMA FIBRONECTIN -2572984 2572984
      HUMAN PLASMA FIBRONECTIN -2605444 2605444
      HUMAN PLASMA FIBRONECTIN -2649247 2649247
      HUMAN PLASMA FIBRONECTIN -2698432 2698432
      HUMAN PLASMA FIBRONECTIN -2716639 2716639
      HUMAN PLASMA FIBRONECTIN -2739430 2739430
      HUMAN PLASMA FIBRONECTIN -2762369 2762369
      HUMAN PLASMA FIBRONECTIN -2776847 2776847
      HUMAN PLASMA FIBRONECTIN -2793657 2793657
      HUMAN PLASMA FIBRONECTIN -2830001 2830001
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN -
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2025973 2025973
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2051848 2051848
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2089399 2089399
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2219299 2219299
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2300456 2300456
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2325036 2325036
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN -2488750 2488750

      References

      Reference overviewPub Med ID
      Comparative studies on amniotic fluid and plasma fibronectins
      Ruoslahti E. et al.
      Biochem. J, 193:295-299 (1981) 1981

      7305927 7305927

      Brochure

      Title
      Human Stem Cell Systems

      Data Sheet

      Title
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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      Life Science Research > Proteins and Enzymes > ECM Proteins for Plate Coating