69830 Human Ig-Primer Set

69830
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      69830-3
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          Description
          OverviewThe Ig-Primer Sets are designed for amplification of immunoglobulin (Ig) variable region cDNAs from human and mouse sources. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR (Larrick 1989, Jones 1991), after which the products may be sequenced directly or cloned. This powerful method enables rapid, precise analysis of hybridomas, myeloma cell progression and heterogeneity, idiotype networks, B-cell clonal ontogeny, and phylogenetic relationships.

          In practice, total RNA is isolated from a B-cell source, such as peripheral blood, lymph node(s), spleen, or hybridoma, and first-strand cDNA is prepared using reverse transcriptase. The primer sets are then used in PCR to specifically amplify the variable regions of light- and heavy-chain cDNAs.

          Please call Technical Service if you need more information regarding individual Ig-Primer Sets.

          Catalogue Number69830
          Brand Family Novagen®
          References
          ReferencesLarrick, J.W., et al. 1989. Biochem. Biophys. Res.
          Comm.
          160, 1250. Jones, S.T. and Bendig, M.M. 1991.
          Biotechnology 9, 88.
          Product Information
          500 pmolHu IgM VH 3ʹ-1 Primer
          500 pmolHu IgG VH 3ʹ-1 Primer
          500 pmolHu Ig VH 5ʹ-A Primer
          500 pmolHu Ig VH 5ʹ-B Primer
          500 pmolHu Ig VH 5ʹ-C Primer
          500 pmolHu Igκ VL 3ʹ-1 Primer
          500 pmolHu Igκ VL 5ʹ-A Primer
          500 pmolHu Igλ VL 3ʹ-1 Primer
          500 pmolHu Igλ VL 5ʹ-A Primer
          10 µlHuman Positive Control PCR Template
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Human Ig-Primer Set SDS

          Title

          Safety Data Sheet (SDS) 

          Human Ig-Primer Set Certificates of Analysis

          TitleLot Number
          69830

          References

          Reference overview
          Larrick, J.W., et al. 1989. Biochem. Biophys. Res.
          Comm.
          160, 1250. Jones, S.T. and Bendig, M.M. 1991.
          Biotechnology 9, 88.

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          Catalog Number Description  
          69831 Mouse Ig-Primer Set Show Pricing & Availability

          Categories

          Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Primers