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SCM026 | Human Embryonic Stem (ES) Cell Embryoid Body Formation Medium

500 mL  Five 100mL bottles
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      Key Specifications Table

      Key Applications
      Catalogue NumberSCM026
      DescriptionHuman Embryonic Stem (ES) Cell Embryoid Body Formation Medium
      OverviewHuman Embryonic Stem (ES) Cell Embryoid Body Formation Medium is a formulation that is designed for the spontaneous or directed differentiation of human embryonic stem cells
      Background InformationEmbryonic stem cells are pluripotent cells derived from the inner cell mass of preimplantation embryos and have the ability to differentiate into cells comprising all three embryonic germ layers. One method to identify the pluripotent potential of hES cells is the induction of in vitro differentiation through the culture of human ES cells in suspension to form embryoid bodies. Embryoid bodies are subsequently evaluated for the presence of markers for ectoderm, mesoderm, and endoderm. Additionally, embryoid bodies are considered to provide an environment that mimics early embryonic development and can hence be used for the differentiation of hES cells towards specific lineages.
      Materials Required but Not Delivered• Cryopreserved Human Embryonic Stem Cells (Catalog Nos. SCC020 and SCC021).
      • HEScGRO MediumTM for Human ES Cell Culture (Catalog No. SCM020).
      • Accumax™ (Catalog No. SCR006).
      • Dulbecco’s Phosphate-Buffered Saline (1X PBS) (Catalog No. BSS-1005-B).
      • Tissue culture plates.
      • Matrigel, 0.1 % gelatin solution, human collagen IV, or other tissue culture plastic coating material.
      • Mitotically inactivated Detroit 551 feeder cells are recommended (ATCC cat no. CCL-110) plated at 60,000 cells/cm2 .
      • Low attachment plates.
      Product Information
      ApplicationThe Human Embryonic Stem (ES) Cell Embryoid Body Formation Medium is a formulation that is designed for the spontaneous or directed differentiation of human embryonic stem cells.
      Key Applications
      • Stem Cell Culture
      Application NotesMillipore’s Human ES Cell Embryoid Body Formation Medium has been optimized and qualified to support the formation of embryoid bodies. The medium can be used to form embryoid bodies in suspension culture on low adhesion plates. Embryoid bodies formed using SCM026 have been shown to facilitate the differentiation of human ES cells into neural, endodermal, and cardiac cell lineages.
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach lot of medium is tested for the ability to support human ES cell embryoid body formation. Sterility Testing: Negative.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsHuman Embryonic Stem Cell Embryoid Body Formation Medium should be stored at -20°C until ready to use. Upon thawing, the medium should be stored at 2-8°C and given a 3 week expiration dating. Dispense into aliquots to avoid repeated heating prior to each use. The media can also be dispensed into aliquots and refrozen at -20°C although repeat freeze-thaws are not recommended.
      Packaging Information
      Material Size500 mL
      Material PackageFive 100mL bottles
      Transport Information
      Supplemental Information

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      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Stem Cell Culture Media