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CC054 | Human Collagen Type III

CC054
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      CULT
      Description
      Catalogue NumberCC054
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHuman Collagen Type III
      OverviewHuman type III collagen purified by serial salt precipitations, alcohol precipitation and DEAE chromatography of an acetic acid extraction of human placenta, following mild pepsin digestion. Composition: [a1(III)]3, native triple helix. Ability to form native helical structure verified by ORD measurement, competence in microfibril formation and reactivity with anti-collagen type-specific monoclonal antibodies
      Alternate Names
      • alpha 1 Type III Collagen
      Background InformationCOL3A1 is the gene responsible for producing the pro-alpha1(III) chain of type III procollagen. Type III collagen, which adds structure and strength to connective tissues, is found in many places in the body, especially skin, lung, intestinal walls, and the walls of blood vessels. Collagen III is initially produced as procollagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the procollagen molecule is modified by the cell. Enzymes modify the amino acids lysine and proline in the protein strands by adding chemical groups that are necessary for the strands to form a stable molecule and then later to crosslink to other molecules outside the cell. Other enzymes add sugars to the protein. The type III procollagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen's tissue strengthening function.{http://ghr.nlm.nih.gov}
      References
      Product Information
      PresentationLiquid in 0.5 M acetic acid, pH 2.5.
      Applications
      Key Applications
      • Cell Culture
      Biological Information
      Concentration1 mg/mL
      PurityHuman collagen type III: 90% pure by SDS-PAGE.

      Human collagen type I: <10%

      Human collagen types II, IV-VI, and non-collagen proteins: <1%

      Retention of native structure was confirmed by ability to form microfibrils.
      SourceHuman placenta, negative for HbsAG, HCV and HIV 1 & 2 antibodies.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]
      Gene Symbol
      • COL3A1
      • EDS4A
      • FLJ34534
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02461 # Collagen type III occurs in most soft connective tissues along with type I collagen.
      SIZE: 1466 amino acids; 138564 Da
      SUBUNIT: Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
      PTM: Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
      DISEASE: SwissProt: P02461 # Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type III (EDS-III) [MIM:130020]; also known as benign hypermobility syndrome. Inheritance is autosomal dominant. EDS is characterized by joint laxity and hyperextensible skin. It is divided into nine different subtypes based on biochemical and clinical variations. & Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type IV (EDS-IV) [MIM:130050]. EDS-IV is the most severe form of the disease, in that it often produces life-threatening consequences, such as rupture of the arteries, bowel, or uterus. A variant form of EDS-IV is Gottron type acrogeria [MIM:201200]. The main characteristics are atrophy and mottled-type hyperpigmentation of the acral skin, resulting in an aged appearance. & Defects in COL3A1 may be a cause of aortic aneurysm [MIM:100070]. Aortic aneurysm consists of a dangerous ballooning of the aorta which is caused by a defect in the artery's wall.
      SIMILARITY: SwissProt: P02461 ## Belongs to the fibrillar collagen family. & Contains 1 VWFC domain.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20ºC in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      HUMAN COLLAGEN TYPE III PURIFIED PROTEIN - 2148198 2148198
      HUMAN COLLAGEN TYPE III PURIFIED PROTEIN - 2398861 2398861
      HUMAN COLLAGEN TYPE III - 2519237 2519237
      HUMAN COLLAGEN TYPE III -2718343 2718343
      HUMAN COLLAGEN TYPE III -2734777 2734777
      HUMAN COLLAGEN TYPE III -2786968 2786968
      HUMAN COLLAGEN TYPE III PURIFIED PROTEIN - 2276290 2276290
      HUMAN COLLAGEN TYPE III PURIFIED PROTEIN - 2295639 2295639

      References

      Reference overviewPub Med ID
      Isolation and characterization of a native placental basement-membrane collagen and its component alpha chains.
      Glanville, R W, et al.
      Eur. J. Biochem., 95: 383-9 (1979) 1979

      Show Abstract
      456357 456357
      Characterization of a novel collagen chain in human placenta and its relation to AB collagen.
      Sage, H and Bornstein, P
      Biochemistry, 18: 3815-22 (1979) 1979

      Show Abstract
      224919 224919
      Physicochemical characterization and molecular organization of the collagen A and B chains
      Rhodes, R K and Miller, E J
      Biochemistry, 17:3442-3448 (1978) 1978

      687595 687595

      Data Sheet

      Title
      Human Collagen Type III - Data Sheet

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      Catalog Number Description  
      CC054-K Human Collagen Type III (1mg) Show Pricing & Availability

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      Life Science Research > Proteins and Enzymes > ECM Proteins for Plate Coating