14-697 Histone H4 Protein, human recombinant, 1 mg

14-697
1 mg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsEntrez Gene NumberUni Prot Number
      Enzyme Assays, WB NM_003538.3 P62805
      Description
      Catalogue Number14-697
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHistone H4 Protein, human recombinant, 1 mg
      OverviewRecombinant human histone H4 produced in E. coli.
      Alternate Names
      • H4
      • Histone H4
      References
      Product Information
      Applications
      ApplicationRecombinant human histone H4 produced in E. coli, purified by FPLC. For use in Enzyme Assays & Western Blotting.
      Key Applications
      • Enzyme Assays
      • Western Blotting
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/C
      • H4/D
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Purification MethodFPLC agarose column
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight11kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated as a substrate for in vitro enzymatic reactions.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Histone H4 Protein, human recombinant, 1 mg SDS

      Title

      Safety Data Sheet (SDS) 

      Histone H4 Protein, human recombinant, 1 mg Certificates of Analysis

      TitleLot Number
      Histone H4, human recombinant2461162
      Histone H4, human recombinant (recombinant protein expressed in E. coli)2874971
      Histone H4, human recombinant (recombinant protein expressed in E. coli) - JBC1893938JBC1893938
      Histone H4, human recombinant - 3033930339
      Histone H4, human recombinant -25415872541587
      recombinant protein expressed in E. coli)_27647132764713

      References

      Reference overviewPub Med ID
      Schizosaccharomyces pombe Hat1 (Kat1) is associated with Mis16 and is required for telomeric silencing.
      Tong, K; Keller, T; Hoffman, CS; Annunziato, AT
      Eukaryotic cell  11  1095-103  2012

      Show Abstract
      22771823 22771823
      Expression and purification of recombinant human histones.
      Tanaka, Yoshinori, et al.
      Methods, 33: 3-11 (2004)  2004

      Show Abstract
      15039081 15039081
      Expression, purification, and structural characterization of human histone H4.
      Vergani, Laura, et al.
      Protein Expr. Purif., 24: 420-8 (2002)  2002

      Show Abstract
      11922758 11922758
      Characterization of nucleosome core particles containing histone proteins made in bacteria.
      Luger, K, et al.
      J. Mol. Biol., 272: 301-11 (1997)  1997

      Show Abstract
      9325091 9325091

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Control Histones