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14-494 | Histone H3, human recombinant

100 µg  also available in larger sizes, please inquire for pricing and availability.
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      Replacement Information

      Key Specifications Table

      Key ApplicationsEntrez Gene NumberUni Prot Number
      EA NM_002107.3 Q16695
      Catalogue Number14-494
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHistone H3, human recombinant
      OverviewRecombinant human histone H3
      Alternate Names
      • H3
      • Histone H3
      Product Information
      PresentationLyophilized powder
      ApplicationRecombinant human histone H3.
      Key Applications
      • Enzyme Assay
      Biological Information
      Sourcehuman histone H3 produced in E. coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • H3F3A
      • H3F3
      • MGC87782
      • H3.3B
      • MGC87783
      • H3F3B
      • H3.3A
      Purification MethodFPLC agarose column
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight~17kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by its use as a substrate for in vitro enzymatic reactions, such as acetylation by PCAF
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at 4°C
      Packaging Information
      Material Size100 µg
      Material Packagealso available in larger sizes, please inquire for pricing and availability.
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Histone H3, human - 24426 24426
      Histone H3, human - 30036 30036
      Histone H3, human - DAM1675445 DAM1675445
      Histone H3, human - DAM1713146 DAM1713146
      Histone H3, human - DAM1764934 DAM1764934


      Reference overviewApplicationPub Med ID
      Aurora kinase-C-T191D is constitutively active mutant.
      Jabbar Khan,Sanaullah Khan,Sobia Attaullah,Ijaz Ali,Shahid Niaz Khan
      BMC cell biology 13 2012

      Show Abstract
      22443468 22443468
      Histone-modifying complexes regulate gene expression pertinent to the differentiation of the protozoan parasite Toxoplasma gondii.
      Saksouk, N; Bhatti, MM; Kieffer, S; Smith, AT; Musset, K; Garin, J; Sullivan, WJ; Cesbron-Delauw, MF; Hakimi, MA
      Molecular and cellular biology 25 10301-14 2005

      Show Abstract Full Text Article
      Western Blotting16287846 16287846

      Technical Info

      Evolving strategies for application specific validation of research use antibodies (EMD)

      Related Products & Applications

      Product Families


      Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Control Histones