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382184 | Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda

382184
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      382184-50UG
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      Stocked 
      Discontinued
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      Available
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          Glass bottle 50 μg
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          Description
          OverviewFull length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag® sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.
          Catalogue Number382184
          Brand Family Calbiochem®
          SynonymsHDAC8, Human, Recombinant
          References
          ReferencesWaltregny, D., et al. 2005. FASEB J. 19, 966.
          Buggy, J.J., et al. 2000. Biochem J. 350, 199
          Product Information
          Unit of DefinitionOne unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.
          FormLiquid
          FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN®-20 detergent, pH 8.0.
          Applications
          ApplicationHistone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda, is a full length HDAC8 fused to His-Tag at the C-terminus. Useful in the study of HDAC8 regulation and for inhibitor screening.
          Biological Information
          Purity≥90% by SDS-PAGE
          Specific Activity≥10 units/µg protein
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          382184

          References

          Reference overview
          Waltregny, D., et al. 2005. FASEB J. 19, 966.
          Buggy, J.J., et al. 2000. Biochem J. 350, 199

          Brochure

          Title
          Biologics 35.1
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-May-2008 JSW
          SynonymsHDAC8, Human, Recombinant
          DescriptionFull length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag® sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.
          FormLiquid
          FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN®-20 detergent, pH 8.0.
          Concentration Label Please refer to vial label for lot-specific concentration
          Recommended reaction conditions
          137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2, pH 8.0, 100 µM fluorescent substrate [Arg-His-Lys(Ac)-Lys(Ac)], 100 µg/ml BSA, 15 ng/ml HDAC8; incubate at 30°C for 60 min.
          Purity≥90% by SDS-PAGE
          Specific activity≥10 units/µg protein
          Unit definitionOne unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesWaltregny, D., et al. 2005. FASEB J. 19, 966.
          Buggy, J.J., et al. 2000. Biochem J. 350, 199