382180 | Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda

382180
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      382180-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewFull length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system. Actively maintained in the cytoplasm, HDAC6 is uniquely dependent on its ubiquitin-binding domain and functions at the nexus between two cellular signaling systems, involving protein acetylation and ubiquitination. The active deacetylase is useful in the study of HDAC6 regulation and for inhibitor screening.
          Catalogue Number382180
          Brand Family Calbiochem®
          SynonymsHDAC6, Human, Recombinant
          References
          ReferencesPandey, U.B., et al. 2007. Nature 447, 859.
          Hubbert, C., et al. 2002. Nature 417, 455.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent HDAC peptide substrate per min at 30°C.
          FormLiquid
          FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 0.05% Tween®-20 detergent, 10% glycerol, pH 8.0.
          Applications
          ApplicationHistone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda, is a full-length HDAC6 fused to GST at the N-terminus. Useful in the study of HDAC6 regulation and for inhibitor screening.
          Biological Information
          Purity≥80% by SDS-PAGE
          Specific Activity≥50 units/µg protein
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          382180

          References

          Reference overview
          Pandey, U.B., et al. 2007. Nature 447, 859.
          Hubbert, C., et al. 2002. Nature 417, 455.

          Brochure

          Title
          Biologics 35.1
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-August-2008 JSW
          SynonymsHDAC6, Human, Recombinant
          DescriptionFull length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system. Actively maintained in the cytoplasm, HDAC6 is uniquely dependent on its ubiquitin-binding domain and functions at the nexus between two cellular signaling systems, involving protein acetylation and ubiquitination. The active deacetylase is useful in the study of HDAC6 regulation and for inhibitor screening.
          FormLiquid
          FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 0.05% Tween®-20 detergent, 10% glycerol, pH 8.0.
          Concentration Label Please refer to vial label for lot-specific concentration
          Recommended reaction conditions
          137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2 0.1 mg/ml BSA, 30 µM fluorescent HDAC substrate, 5 ng/µl HDAC6; incubate at 30° for 60 min.
          Purity≥80% by SDS-PAGE
          Specific activity≥50 units/µg protein
          Unit definitionOne unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent HDAC peptide substrate per min at 30°C.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesPandey, U.B., et al. 2007. Nature 447, 859.
          Hubbert, C., et al. 2002. Nature 417, 455.