70796 His•Tag® Monoclonal Antibody

70796
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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      70796-3
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          Plastic ampoule 100 μg
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          70796-4
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              Plastic ampoule 3 μg
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              Description
              Overview

              The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of the Novagen® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10-8 to 1 × 10-9 M) enables sensitive, specific detection of His•Tag fusion proteins at antibody concentrations of 0.1-0.2 µg/ml. The His•Tag Monoclonal Antibody binds to N-terminal, C-terminal, and internal His•Tag sequences. This antibody also detects the recombinant marker bands in the Perfect Protein™ and Trail Mix™ Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-µg package size provides enough purified antibody for 1000 ml of working solution.

              * Not recommended for HRP-based detection of Trail Mix Western Markers.



              Catalogue Number70796
              Brand Family Novagen®
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              Product Information
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              Biological Information
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              Safety Information according to GHS
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              Storage and Shipping Information
              Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
              Toxicity Standard Handling
              Storage +2°C to +8°C
              Do not freeze Ok to freeze
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              Supplemental Information
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              Documentation

              His•Tag® Monoclonal Antibody SDS

              Title

              Safety Data Sheet (SDS) 

              His•Tag® Monoclonal Antibody Certificates of Analysis

              TitleLot Number
              70796

              Brochure

              Title
              Protein Purification and Detection Tools

              Citations

              Title
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            • User Protocols

              Title
              TB283 His•Tag® Monoclonal Antibody