375228 | Hepatocyte Growth Factor, Human, Recombinant, S. frugiperda

375228
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥95% by SDS-PAGE

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      375228-5UG
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      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
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          Plastic ampoule 5 μg
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          Description
          OverviewRecombinant, human hepatocyte growth factor expressed in S. frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.
          Catalogue Number375228
          Brand Family Calbiochem®
          SynonymsrhHGF
          References
          ReferencesKaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
          Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
          Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
          Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
          Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.
          Product Information
          FormLyophilized
          FormulationLyophilized from a sterile-filtered solution in 20 mM Sodium Phosphate, 0.5 M NaCl, 50 µg BSA/µg HGF, pH 7.0.
          Applications
          Biological Information
          Biological activityED₅₀ = 20-40 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in an HGF-responsive epithelial cell line, 4MBr-5
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          Contaminantsendotoxin: ≤100 pg/µg HGF
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for up to 3 months at -70°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          375228

          References

          Reference overview
          Kaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
          Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
          Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
          Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
          Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.

          Brochure

          Title
          Growth Factors and Cytokines Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-August-2009 JSW
          SynonymsrhHGF
          DescriptionRecombinant, human hepatocyte growth factor expressed in Spodoptera frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.
          FormLyophilized
          FormulationLyophilized from a sterile-filtered solution in 20 mM Sodium Phosphate, 0.5 M NaCl, 50 µg BSA/µg HGF, pH 7.0.
          Purity≥95% by SDS-PAGE
          Contaminantsendotoxin: ≤100 pg/µg HGF
          Biological activityED₅₀ = 20-40 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in an HGF-responsive epithelial cell line, 4MBr-5
          SolubilityReconstitute to a concentration of ≥1 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for up to 3 months at -70°C.
          Toxicity Standard Handling
          ReferencesKaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
          Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
          Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
          Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
          Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.