71493 HRV 3C Protease

71493
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      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      71493-10000UN
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          Glass bottle 10000 un
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          71493-3
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              Glass bottle 500 u
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              Description
              OverviewRecombinant type 14 3C protease from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. HRV 3C is a highly purified recombinant 6XHis-fusion protein, that recognizes the same cleavage site as the native enzyme: LeuGluValLeuPheGln↓GlyPro. The small, 22-kDa size of the protease, optimal activity at 4°C, high specificity, and His•Tag® fusion make HRV 3C protease an ideal choice for rapid removal of fusion tags. The pET expression vectors pET-47b(+) through pET-50b(+) incorporate the HRV 3C protease cleavage site in combinations with His•Tag, S•Tag™, thioredoxin (Trx•Tag™), glutathione-S-transferase (GST•Tag™), and NusA (Nus•Tag™) sequences. The combination of pET-47b(+) to pET-50b(+) vectors for expression, HRV 3C protease for fusion tag cleavage, and Ni-NTA His•Bind® metal affinity chromatography for protein purification as well as protease and fusion tag removal allows production of recombinant proteins free of vector-encoded sequences. Other expression vectors, including the pET-52b(+) vector, the InsectDirect™ pIEx™-9 vector, and the multisystem pTriEx™-6 vector, all incorporate the HRV 3C protease cleavage site in combination with the Strep•Tag® II and His•Tag sequences. Supplied as: 1.8-3.0 U/µl in 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.

              Catalogue Number71493
              Brand Family Novagen®
              References
              Product Information
              Activity1.8-3.0 U/µl
              Unit of DefinitionOne unit will cleave >95% of 100 µg His•Tag fusion control protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h.
              500 UHRV 3C Protease
              10 µgHRV 3C Cleavage Control Protein
              10 ml10X HRV 3C Cleavage Buffer
              FormulationIn 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 0.5 mM THP, and 50% glycerol.
              Applications
              Biological Information
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              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Shipped with Blue Ice or with Dry Ice
              Toxicity Standard Handling
              Storage -20°C
              Do not freeze Ok to freeze
              Packaging Information
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              Supplemental Information
              Specifications

              Documentation

              HRV 3C Protease SDS

              Title

              Safety Data Sheet (SDS) 

              HRV 3C Protease Certificates of Analysis

              TitleLot Number
              71493

              Brochure

              Title
              Bulk packaging
              Protein Purification and Detection Tools
              Protein Purification and Preparation

              Citations

              Title
            • Yanqiu Yuan, et al. (2007) Crystal structure of a peptidoglycan glycosyltransferase suggests a model for processive glycan chain synthesis. Procedings of the National Academy of Science 104, 5348-5353.
            • User Protocols

              Title
              TB420