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69453 | HMS174(DE3) Competent Cells - Novagen

69453
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      69453-3
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          Glass bottle 0.4 ml
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          69453-4
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              Glass bottle 1 ml
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              Description
              OverviewHMS174 strains provide the recA mutation in a K-12 background. Like BLR, these strains may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

              DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
              Genotype: F- recA1 hsdR(rK12- mK12+) (DE3) (Rif R)
              Catalogue Number69453
              Brand Family Novagen®
              References
              Product Information
              Components
              Guaranteed efficiencyGuaranteed efficiency: > 5 x 10⁶ cfu/µg
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage ≤ -70°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              69453

              Brochure

              Title
              Competent Cell Brochure

              Citations

              Title
            • Monika Walter, et al. (2008) Structure of the receptor-binding protein of bacteriophage Det7: a podoviral tail spike in a myovirus. Journal of Virology 82, 2265-2273.
            • Ishara Azmi, et al. (2006) Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta1. Journal of Cell Biology 172, 705-717.
            • Felice Arcuri, et al. (2005) The translationally controlled tumor protein is a novel calcium binding protein of the human placenta andregulates calcium handling in trophoblast cells. Biology of Reproduction 73, 745-751.
            • Helen N. Murphy, et al. (2005) The OtsAB pathway is essential for trehalose biosynthesis in Mycobacterium tuberculosis. Journal of Biological Chemistry 280, 14524-14529.
            • Ken Okada and Toshiharu Hase. (2005) Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1. Journal of Biological Chemistry 280, 60672-20679.
            • James B. Thoden and Hazel M. Holden. (2005) The molecular architecture of galactose mutarotase/UDP-galactose 4-epimerase from Saccharomyces cerevisiae. Journal of Biological Chemistry 280, 21900-21907.
            • Stacey A. L. Tom-Yew, et al. (2005) Anion-independent iron coordination by the Campylobacter jejuni ferric binding protein. Journal of Biological Chemistry 280, 9283-9290.
            • Sharon Yagur-Kroll and Orna Amster-Choder. (2005) Dynamic membrane topology of the E. coli -glucoside transporter β-glucosidase transporter BglF. Journal of Biological Chemistry 280, 19306-19318.
            • Bin Zhao, et al. (2005) Binding of two flaviolin substrate molecules, oxidative coupling, and crystal structure of Streptomyces coelicolor A3(2) cytochrome P450 158A2. Journal of Biological Chemistry 280, 11599-11607.
            • B. Huang, et al. (1996) NMR structure and mutagenesis of the Fas (APO-1/CD95) death domain. 384, 638-641.
            • Ramavedi Gudi, et al. (1995) Diversity of the pyruvate dehydrogenase kinase gene family in humans. Journal of Biological Chemistry 270, 28989-28994.
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              Categories

              Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression