|Description||Guava Nexin Reagent for Flow Cytometry - 100 tests|
|Overview||The Guava Nexin® Annexin V Assay offers a simple, reproducible, and reliable assessment of early apoptosis. The assay relies on the translocation of phosphatidyl serine (PS) to the outer surface of the cell membrane, an event often associated with the onset of apoptosis.
Simple, sensitive, and reproducible
A mix-and-read assay for monitoring externalization of PS through the binding of Annexin V to the exposed PS, the automated single-cell analysis assay is the choice for monitoring apoptosis due to its sensitivity and reproducibility. Annexin V is a calcium-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), a membrane component normally localized to the internal face of the cell membrane. Early in the apoptotic pathway, molecules of PS are translocated to the outer surface of the cell membrane where Annexin V can readily bind to them.
The assay relies on a two-dye stategy: 1) Annexin V-PE to detect PS on the external membrane of apoptotic cells and 2) 7-AAD, a cell impermeant dye, as an indicator of membrane structural integrity. 7-AAD is excluded from live, healthy cells and early apoptotic cells, but permeates late-stage apoptotic and dead cells. For use with all instruments. Click here for more information regarding Instrument Compatibility
|Application||The Guava Nexin Annexin V Assay offers a simple, reproducible & reliable assessment of early apoptosis.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Material Size||100 Tests|
Guava Nexin Reagent for Flow Cytometry - 100 tests SDS
|Reference overview||Application||Species||Pub Med ID|
|Rational incorporation of selenium into temozolomide elicits superior antitumor activity associated with both apoptotic and autophagic cell death.|
Cheng, Yan, et al.
PLoS ONE, 7: e35104 (2012) 2012
The DNA alkylating agent temozolomide (TMZ) is widely used in the treatment of human malignancies such as glioma and melanoma. On the basis of previous structure-activity studies, we recently synthesized a new TMZ selenium analog by rationally introducing an N-ethylselenocyanate extension to the amide functionality in TMZ structure.
|Aberrant Expression of OX1 Receptors for Orexins in Colon Cancers and Liver Metastases: an Openable Gate to Apoptosis.|
Voisin, Thierry, et al.
Cancer Res., 71: 3341-51 (2011) 2011
Resistance to apoptosis is a recurrent theme in colon cancer. We have shown previously that the 7-transmembrane spanning receptor OX1R for orexins promotes robust apoptosis in the human colon cancer cell line HT29 through an entirely novel mechanism involving phosphorylation of tyrosine-based motifs in OX1R. Here, we investigated the status of OX1R in a large series of human colorectal tumors and hepatic metastases. All primary colorectal tumors regardless of their localization and Duke's stages and all hepatic metastases tested expressed OX1R mRNA and/or protein. In sharp contrast, adjacent normal colonocytes or hepatocytes as well as control normal tissues were negative. Next, we showed that nine human colon cancer cell lines established from primary tumors or metastases expressed OX1R mRNA and underwent important apoptosis on orexin-A challenge. Most interestingly, orexin-A also promoted robust apoptosis in cells that are resistant to the most commonly used drug in colon cancer chemotherapy, 5-fluorouracil. When human colon cancer cells were xenografted in nude mice, orexin-A administered at day 0 strongly slowed the tumor growth and even reversed the development of established tumors when administered 7 days after cell inoculation. Orexin-A also acts by promoting tumor apoptosis in vivo because caspase-3 is activated in tumors on orexin treatment of nude mice. These findings support that OX1R is an Achilles heel of colon cancers, even after metastasis or chemoresistance. They suggest that OX1R agonists might be novel candidates for colon cancer therapy. Cancer Res; 71(9); 3341-51. ©2011 AACR.
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