368067 | Granzyme B Substrate VIII, Colorimetric - Calbiochem

368067
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      368067-5MG
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          Plastic ampoule 5 mg
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          Description
          OverviewA colorimetric substrate for granzyme B (kcat/Km = 6.6 x 104 M-1s-1). Cleavage of pNA is monitored colorimetrically at ~405 nm (ε = 9,160 M-1cm-1).
          Catalogue Number368067
          Brand Family Calbiochem®
          SynonymsAc-IEPD-pNA
          References
          ReferencesHarris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
          Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
          Product Information
          ATP CompetitiveN
          FormWhite lyophilized solid
          Hill FormulaC₂₈H₃₈N₆O₁₁
          Chemical formulaC₂₈H₃₈N₆O₁₁
          ReversibleN
          Applications
          Biological Information
          Primary TargetColorimetric substrate for granzyme B
          Primary Target IC<sub>50</sub>kcat/Km = 6.6 x 104 M⁻¹ sec⁻¹ as substrate for granzyme B
          Purity≥95% by HPLC
          Physicochemical Information
          Cell permeableN
          Peptide SequenceAc-Ile-Glu-Pro-Asp-pNA
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          368067

          References

          Reference overview
          Harris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
          Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-July-2008 RFH
          SynonymsAc-IEPD-pNA
          DescriptionA colorimetric substrate for granzyme B (kcat/Km = 66,600 M-1s-1). Cleavage of pNA is monitored as an increase in absorbance at ~405 nm (ε = 9,160 M-1cm-1).
          FormWhite lyophilized solid
          Intert gas (Yes/No) Packaged under inert gas
          Recommended reaction conditions0.25 µM substrate, 100 mM HEPES buffer, pH 7.5, and 10 mM DTT. Note: optimal enzyme concentration and incubation time should be determined empirically for each assay.
          Chemical formulaC₂₈H₃₈N₆O₁₁
          Peptide SequenceAc-Ile-Glu-Pro-Asp-pNA
          Purity≥95% by HPLC
          SolubilityDMSO (5 mg/ml)
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesHarris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
          Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.