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234374 | Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli

234374
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      234374-10UG
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      Stocked 
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      Available
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          Glass bottle 10 μg
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          Description
          OverviewRecombinant, mouse granulocyte macrophage colony-stimulating factor expressed in E. coli. Pleiotropic cytokine that can stimulate the proliferation, maturation, and function of hematopoietic cells. Reported to improve the response rate to antibiotic therapy in cancer subjects. Activates JAK2 in a variety of cells.
          Catalogue Number234374
          Brand Family Calbiochem®
          SynonymsrmGM-CSF
          References
          ReferencesAnaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
          Watanabe, S., et al. 1996. J. Biol. Chem. 271, 12681.
          Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
          Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
          Gough, N.M., et al. 1985. EMBO J. 4, 645.
          Product Information
          FormLyophilized
          FormulationLyophilized from sterile-filtered PBS containing 50 µg BSA/µg GM-CSF.
          Applications
          ApplicationGranulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli, is a pleiotropic cytokine that can stimulate the proliferation, maturation, and function of hematopoietic cells.
          Biological Information
          Biological activityED₅₀ = 100-300 pg/ml as measured in a cell proliferation assay using a factor-dependent cell line, DA-3
          Purity>97% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤1.0 EU/µg GM-CSF
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 3 months at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          234374

          References

          Reference overview
          Anaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
          Watanabe, S., et al. 1996. J. Biol. Chem. 271, 12681.
          Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
          Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
          Gough, N.M., et al. 1985. EMBO J. 4, 645.

          Brochure

          Title
          Growth Factors and Cytokines Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision29-April-2008 RFH
          SynonymsrmGM-CSF
          DescriptionRecombinant, mouse granulocyte macrophage colony-stimulating factor expressed in E. coli. Pleiotropic cytokine that stimulates the proliferation, maturation, and function of hematopoietic cells. Reported to improve the response rate to antibiotic therapy in cancer subjects. Activates JAK2 in a variety of cells. Target cells include macrophages, granulocytes, and eosinophils. GM-CSF is produced by many cell types, including endothelial cells, fibroblasts, and T-lymphocytes. It can be extracted from most tissues in concentrations exceeding that of serum levels. While many cells produce GM-CSF constitutively, levels of this lymphokine increase rapidly in response to endotoxin, phorbol esters, and certain antigens.
          FormLyophilized
          FormulationLyophilized from sterile-filtered PBS containing 50 µg BSA/µg GM-CSF.
          Purity>97% by SDS-PAGE
          ContaminantsEndotoxin: ≤1.0 EU/µg GM-CSF
          Biological activityED₅₀ = 100-300 pg/ml as measured in a cell proliferation assay using a factor-dependent cell line, DA-3
          SolubilityReconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 3 months at 4°C.
          Toxicity Standard Handling
          Merck USA index14, 4538
          ReferencesAnaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
          Watanabe, S., et al. 1996. J. Biol. Chem. 271, 12681.
          Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
          Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
          Gough, N.M., et al. 1985. EMBO J. 4, 645.