A part of MilliporeSigma

AP202P | Goat Anti-Rat light chain Antibody, HRP conjugate

AP202P
0.5 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberAP202P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionGoat Anti-Rat light chain Antibody, HRP conjugate
      Background InformationLight chains covalently bound to a pair of heavy chains to form an antibody molecule. Immunoglobulin light chains occur in two types, kappa or lambda. Light chain's mass is about 25,000 Daltons per mole. Each light chain consists of about 250 amino-acids long. These are usually designated by the Greek letters kappa and lambda. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
      References
      Product Information
      FormatHRP
      PresentationPurified by immunoaffinity chromatography. Lyophilized in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. 15 mg/mL BSA as stabilizer.
      Applications
      ApplicationGoat anti-Rat light chain Antibody, HRP conjugate is an antibody against light chain for use in ELISA, WB, IC, IH.
      Key Applications
      • ELISA
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesWestern Blotting: 1:5,000 -1:100,000 with chromogenic substrates
      1:10,000 -1:200,000 with ECL substrates
      ELISA: 1:5,000 -1:100,000
      Immunohistochemistry: 1:500 - 1:5,000
      Immunocytochemistry: 1:500 - 1:5,000
      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPrepared from purified rat IgG light chain.
      EpitopeKappa light chain
      HostGoat
      SpecificityThe antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, mouse, human, rabbit, and sheep immunoglobulins.
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteMinimal cross-reaction with bovine, goat, horse, mouse, human, rabbit, and sheep.
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoAffinity Purified
      Molecular Weight25 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2°-8°C for up to 12 months. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.
      Packaging Information
      Material Size0.5 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED POLYCLONAL ANTIBODY - 2123116 2123116
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED POLYCLONAL ANTIBODY - 2154063 2154063
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED POLYCLONAL ANTIBODY - 2446727 2446727
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED -2634189 2634189
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED -2637675 2637675
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED -2665037 2665037
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED -2683232 2683232
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED -2749167 2749167
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED -2751181 2751181
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED -2794788 2794788
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED POLYCLONAL ANTIBODY - 2038256 2038256
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED POLYCLONAL ANTIBODY - 2064687 2064687
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED POLYCLONAL ANTIBODY - 2069350 2069350
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED POLYCLONAL ANTIBODY - 2108471 2108471
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED POLYCLONAL ANTIBODY - 2293685 2293685

      References

      Reference overviewPub Med ID
      Tertiary lymphoid structures are associated with higher tumor grade in primary operable breast cancer patients.
      Figenschau, SL; Fismen, S; Fenton, KA; Fenton, C; Mortensen, ES
      BMC cancer 15 101 2015

      Show Abstract
      25884667 25884667
      Characterisation and prognostic value of tertiary lymphoid structures in oral squamous cell carcinoma.
      Wirsing, AM; Rikardsen, OG; Steigen, SE; Uhlin-Hansen, L; Hadler-Olsen, E
      BMC clinical pathology 14 38 2014

      Show Abstract
      25177210 25177210

      Data Sheet

      Title
      GOAT ANTI-RAT LIGHT CHAIN SPECIFIC HRP CONJUGATED POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Secondary Antibodies