A part of MilliporeSigma

AP183SD | Goat Anti-Rat IgG (H+L) Antibody, DyLight® 649

0.5 mg  
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      Replacement Information
      Catalogue NumberAP183SD
      Trade Name
      • Chemicon
      DescriptionGoat Anti-Rat IgG (H+L) Antibody, DyLight® 649
      Background InformationDyLight™649-conjugated AffiniPure Goat Anti-Rat IgG (H+L) (minimal cross-reaction to bovine, mouse, horse, human, rabbit serum proteins).
      Product Information
      PresentationLyophilized in buffer containing 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA (IgG-Free, Protease-Free) and 0.05% sodium azide.
      ApplicationGoat anti-Rat IgG (H+L) Antibody, DyLight 649 is an antibody against Rat IgG (H+L) for use in 0.
      Key Applications
      • Immunofluorescence
      Application NotesFluorophore Information
      DyLight™649-TFP ester
      Amax=652; Emax=670
      Fluorophore/Protein Absorbance Ratio: A652/A280=4.7
      Biological Information
      Concentration0.5 mg
      SpecificityBased on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
      Species Reactivity
      • Rat
      Species Reactivity NoteThe antibody has been tested by ELISA and/or solid-phase absorbed to ensure minimal cross-reaction with mouse, human, bovine, horse, and rabbit serum proteins, but may cross-react with immunoglobulins from other species.
      Antibody TypePolyclonal Antibody
      Purification MethodThe antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceReconstitute the product to 1.3 mg/mL with dH2O.
      Suggested dilution range for most applications: 1:100 – 1:800.
      Optimal working dilutions must be determined by the end user.
      Usage Statement
      • Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC lyophilized from date of receipt.
      After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size0.5 mg
      Transport Information
      Supplemental Information