A part of MilliporeSigma

AP200A | Goat Anti-Mouse light chain Antibody, Alkaline Phosphatase conjugate

0.5 mg  
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      Replacement Information
      Catalogue NumberAP200A
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionGoat Anti-Mouse light chain Antibody, Alkaline Phosphatase conjugate
      Background InformationAntibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
      Product Information
      FormatAlkaline Phosphatase
      PresentationLyophilized from a solution in 0.01M Tris-HCl, 0.25M NaCl, pH 8.0, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer.Purified by immunoaffinity chromatography.
      ApplicationGoat anti-Mouse light chain Antibody, Alkaline Phosphatase conjugate detects level of Mouse light chain & has been published & validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesSuggested dilutions:
      Western Blotting: 1:5,000 -1:50,000 of the 1 mg/mL stock.
      ELISA: 1:5,000 -1:50,000 of the 1 mg/mL stock.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPrepared from purified mouse IgG light chain.
      EpitopeKappa light chain
      SpecificityThe antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep immunoglobulins.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMinimal cross-reaction with bovine, goat, horse, human, rabbit, rat, and sheep.
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoAffinity Purified
      Molecular Weight25 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.4 ml water will yield a final concentration of 1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.
      Packaging Information
      Material Size0.5 mg
      Transport Information
      Supplemental Information

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      Alternative Format

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      AP200P Goat Anti-Mouse light chain Antibody, HRP conjugate Show Pricing & Availability
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      Life Science Research > Antibodies and Assays > Secondary Antibodies