354406 Glutaredoxin-S2, E. coli

354406
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      354406-1MG
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          Plastic ampoule 1 mg
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          Description
          OverviewNative glutaredoxin-S2 isolated from E. coli. Glutaredoxin functions as a glutathione-dependent hydrogen donor for ribonucleotide reductase. It is useful as a general disulfide reductant for the in vitro study of protein folding mechanisms and has been demonstrated to work in conjunction with protein disulfide isomerase to enhance refolding of scrambled RNase A and RNase T1.
          Catalogue Number354406
          Brand Family Calbiochem®
          SynonymsGrx1
          References
          ReferencesRuoppolo, M., et al. 1997. Biochemistry 36, 12259.
          Sun, C., et al. 1997. Protein Sci. 6, 383.
          Prinz, W.A., et al. 1997. J. Biol. Chem. 272, 15661.
          Holmgren, A. and Alund, F. 1995 Methods Enzymol. 252, 283.
          Hoog, J.O., et al. 1986. Gene 43, 13.
          Product Information
          Unit of DefinitionUnits are defined using a standard HED assay.
          FormLyophilized
          FormulationLyophilized from 0.5% NH₄HCO₃.
          Applications
          ApplicationGlutaredoxin-S2, E. coli, acts as a glutathione-dependent hydrogen donor for ribonucleotide reductase. Useful as a general disulfide reductant for the in vitro study of protein folding mechanisms.
          Biological Information
          Specific Activity≥175 µmole NADPH oxidized/min/mg
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          354406

          References

          Reference overview
          Ruoppolo, M., et al. 1997. Biochemistry 36, 12259.
          Sun, C., et al. 1997. Protein Sci. 6, 383.
          Prinz, W.A., et al. 1997. J. Biol. Chem. 272, 15661.
          Holmgren, A. and Alund, F. 1995 Methods Enzymol. 252, 283.
          Hoog, J.O., et al. 1986. Gene 43, 13.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-July-2008 RFH
          SynonymsGrx1
          DescriptionNative glutaredoxin-S2 isolated from E. coli. Glutaredoxin functions as a glutathione-dependent hydrogen donor for ribonucleotide reductase. It is useful as a general disulfide reductant for the in vitro study of protein folding mechanisms and has been demonstrated to work in conjunction with protein disulfide isomerase to enhance refolding of scrambled RNase A and RNase T1.
          FormLyophilized
          FormulationLyophilized from 0.5% NH₄HCO₃.
          Specific activity≥175 µmole NADPH oxidized/min/mg
          Unit definitionUnits are defined using a standard HED assay.
          SolubilityReconstitute in 1 ml 50 mM Tris-HCl, 1 mM EDTA, pH 7.5 to yield a final concentration of 1 mg/ml.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
          Toxicity Standard Handling
          ReferencesRuoppolo, M., et al. 1997. Biochemistry 36, 12259.
          Sun, C., et al. 1997. Protein Sci. 6, 383.
          Prinz, W.A., et al. 1997. J. Biol. Chem. 272, 15661.
          Holmgren, A. and Alund, F. 1995 Methods Enzymol. 252, 283.
          Hoog, J.O., et al. 1986. Gene 43, 13.