361524 | GSK-3β, His•Tag®, Human, Recombinant, E. coli

361524
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      361524-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecombinant, human GSK-3β fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. A dual specificity kinase that plays important roles in insulin- and Wnt-mediated cellular signalings. In AD (Alzheimer’s disease), GSK3β has a major role in microtubule stability by its ability to phosphorylate the microtubule associated protein tau. Its known substrates include glycogen synthase, p90rsk, Tau, c-Jun, and CREB. MW: 53,000-56,000.
          Catalogue Number361524
          Brand Family Calbiochem®
          SynonymsGSK-3β, Tau Protein Kinase I, TPK I
          References
          ReferencesHarwood, A., and Braga, V.M. 2003. Nat. Cell Biol. 5, 275.
          Bhat, R.V., and Budd, S.L. 2002. Neurosignals 11, 251.
          Product Information
          FormSolid
          FormulationLyophilized from 0.1 M NH₄CO₃.
          Applications
          ApplicationGSK-3β, His-Tag®, Human, Recombinant, E. coli, is a dual specificity kinase that plays important roles in insulin- and Wnt-mediated signaling. It is inactivated by phosphorylation at Ser9.
          Biological Information
          Biological activity1 µg GSK-3β maximally phosphorylates 100 ng Tau protein in 30 min in an in vitro kinase assay at 30°C, pH 7.5
          Purity≥90% by SDS-PAGE
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          361524

          References

          Reference overview
          Harwood, A., and Braga, V.M. 2003. Nat. Cell Biol. 5, 275.
          Bhat, R.V., and Budd, S.L. 2002. Neurosignals 11, 251.

          Brochure

          Title
          Biologics 32.4 ( 1.19 MB )
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-January-2009 JSW
          SynonymsGSK-3β, Tau Protein Kinase I, TPK I
          DescriptionRecombinant, human GSK-3β fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. GSK-3β is a dual-specificity kinase that is important role in insulin- and Wnt-mediated cellular signaling events. In Alzheimer's disease GSK-3β is reported to affect microtubule stability via the phosphorylation of microtubule associated protein, Tau. Other known substrates include glycogen synthase, p90rsk, c-Jun, and CREB. MW: 53,000-56,000
          FormSolid
          FormulationLyophilized from 0.1 M NH₄CO₃.
          Recommended reaction conditions25 mM Tris-HCl, pH 7.5, 5 mM β-glycerophosphate, 12 mM MgCl2, 2 mM DTT, 100 µM Na3VO4, 200 µM ATP, 30°C.
          Purity≥90% by SDS-PAGE
          Biological activity1 µg GSK-3β maximally phosphorylates 100 ng Tau protein in 30 min in an in vitro kinase assay at 30°C, pH 7.5
          SolubilityReconstitute in sterile PBS to a final concentration of 1 mg/ml.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
          Toxicity Standard Handling
          ReferencesHarwood, A., and Braga, V.M. 2003. Nat. Cell Biol. 5, 275.
          Bhat, R.V., and Budd, S.L. 2002. Neurosignals 11, 251.