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TR-1001 | GPT Selection Reagent, 500X Mycophenolic Acid; 100X Aminopterin

1 kit  
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      Replacement Information
      Catalogue NumberTR-1001
      Trade Name
      • Specialty Media
      DescriptionGPT Selection Reagent, 500X Mycophenolic Acid; 100X Aminopterin
      OverviewMycophenolic Acid is an antibiotic useful in research for the selection of animal cells that express the E. coli gene coding for XGPRT (xanthine guanine phosphoribosyltransferase). Mycophenolic Acid is also used as immunosuppressive agent becasue it acts by inhibiting lymphocyte proliferation and antibody production.

      Cells transfected with the E. coli GPT gene can be selected for growth in medium containing GPT Selection Reagent.

      Each Kit Contains:
      • 500X Mycophenolic Acid containing ETOH (12.5mg/mL) 2 x 1mL

      • 1 bottle (10mL) of 100X Aminopterin solution which consists of:
      - xanthine 25 mg
      - hypoxanthine: 1.5 mg
      - aminopterin: 0.2mg
      - 0.5M NaOH: 1mL vial
      Alternate Names
      • MPA & Aminopterin solution
      Product Information
      Component mg/mL
      NaOH 20
      Hypoxanthine 1.5
      Thymidine 1
      Xanthine 25
      Aminopteron 0.2
      Mycophen. Acid 12.5
      ApplicationThe Mycophenolic Acid is an antibiotic useful in research for the selection of animal cells that express the E. coli.
      Application Notes1. Seed approximately 106 cells on 100 mm culture plates in Dulbecco's modified Eagle medium (DMEM) containing 5% fetal calf serum and penicillin/streptomycin, and allow them to grow for twenty-four hours.

      2. Transfect the culture with 10-20 μg of the plasmid-EcoGPT DNA.

      3. After 3 days at 37°C in DMEM containing 5% fetal calf serum, trypsinize the cell monolayers and disperse 5 x 105 cells on fresh 100 mm culture plates in DMEM containing 10% dialyzed fetal calf serum supplemented with the MPA and Aminopterin solutions. This will give a final solution of approximately: 250 μg/mL xanthine, 15 μg/mL thymidine, 2 μg/mL aminopterin, and 25 μg/mL mycophenolic acid. Dialyzed serum is used to speed the selection process. Selection will be slower with undialyzed serum.

      4. Twenty-four hours later, replace the medium with fresh medium containing the same supplements, and fluid change.

      5. Repeat every 3 days. Colonies are visible in 7-10 days, typically.

      Note: Other media bases can also be used such as Ham's F12. Much depends upon the cell type being used.
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information