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509227 | G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 108321-42-2 - Calbiochem

509227
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₀H₄₀N₄O₁₀ · 2H₂SO₄ 108321-42-2

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      5.09227.0001
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          Glass bottle 20 ml
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          Description
          OverviewThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml
          Catalogue Number509227
          Brand Family Calbiochem®
          SynonymsG418, G 418
          References
          ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
          Jimenez, A., and Davies, J. 1980. Nature 287, 869.
          Product Information
          CAS number108321-42-2
          FormLiquid
          FormulationSterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in PBS, pH 7.
          Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
          Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
          Applications
          Biological Information
          Purity≥98% by TLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          S PhraseS: 36/37/39

          Wear suitable protective clothing, gloves and eye/face protection.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          References

          Reference overview
          Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
          Jimenez, A., and Davies, J. 1980. Nature 287, 869.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-December-2014 JSW
          SynonymsG418, G 418
          DescriptionThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml
          FormLiquid
          FormulationSterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in PBS, pH 7.
          Intert gas (Yes/No) Packaged under inert gas
          CAS number108321-42-2
          Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
          Purity≥98% by TLC
          Storage +2°C to +8°C
          Protect from light
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.
          Toxicity Standard Handling
          ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
          Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
          Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
          Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
          Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
          Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
          Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
          Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
          Jimenez, A., and Davies, J. 1980. Nature 287, 869.