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345810 | G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

345810
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₀H₄₀N₄O₁₀ · 2H₂SO₄ 108321-42-2

      Pricing & Availability

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      345810-100GM
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          345810-1GM
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              Glass bottle 1 gm
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              345810-250MG
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                  345810-25GM
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                      345810-500MG
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                          345810-5GM
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                              Description
                              OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
                              Also available as a sterile-filtered aqueous solution (Cat. No. 345812), a sterile filtered solution in 100 mM HEPES (Cat. No. 508940), and a sterile-filtered solution PBS (Cat. No. 509227).
                              Catalogue Number345810
                              Brand Family Calbiochem®
                              SynonymsNMD Inhibitor I, G418, G 418
                              References
                              ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                              Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                              Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                              Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                              Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                              Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                              Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                              Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                              Jimenez, A., and Davies, J. 1980. Nature 287, 869.
                              Product Information
                              CAS number108321-42-2
                              FormWhite powder
                              Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                              Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                              Structure formula Image
                              Applications
                              Biological Information
                              Biological activityAssay: ≥730 µg/mg
                              Purity≥98% by TLC
                              Physicochemical Information
                              ContaminantsAmmonia: ≤1.0%; heavy metals: ≤100 ppm
                              Dimensions
                              Materials Information
                              Toxicological Information
                              Safety Information according to GHS
                              RTECSCB9378500
                              Safety Information
                              Product Usage Statements
                              Storage and Shipping Information
                              Ship Code Ambient Temperature Only
                              Toxicity Standard Handling
                              Storage +15°C to +30°C
                              Do not freeze Ok to freeze
                              Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.
                              Packaging Information
                              Transport Information
                              Supplemental Information
                              Specifications

                              Documentation

                              SDS

                              Title

                              Safety Data Sheet (SDS) 

                              Certificates of Analysis

                              TitleLot Number
                              345810

                              References

                              Reference overview
                              Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                              Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                              Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                              Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                              Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                              Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                              Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                              Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                              Jimenez, A., and Davies, J. 1980. Nature 287, 869.

                              Brochure

                              Title
                              Antibiotics Profiler
                              Biologics 35.1
                              G418/Hygromycin B Flyer
                              G418/Hygromycin B Flyer

                              Citations

                              Title
                            • Ellen F. Cohn, et al. (2006) Abrupt Expression of TLR4 in TLR4-Deficient Macrophages Imposes a Selective Disadvantage: Genetic Evidence for TLR4-Dependent Responses to Endogenous, Nonmicrobial Stimuli. Journal of Immunology 176, 1185-1194.
                            • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
                            • Data Sheet

                              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                              Revision01-December-2011 JSW
                              SynonymsNMD Inhibitor I, G418, G 418
                              DescriptionAminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
                              FormWhite powder
                              Recommended reaction conditionsThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 mg/ml Mammalian cells: 0.1-2.0 mg/ml A multiplying cell will be affected by the presence of G418 sooner than a resting cell. It will take at least two cell generations to achieve cell death in sensitive cell lines.
                              CAS number108321-42-2
                              RTECSCB9378500
                              Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
                              Structure formula
                              Purity≥98% by TLC
                              ContaminantsAmmonia: ≤1.0%; heavy metals: ≤100 ppm
                              Biological activityAssay: ≥730 µg/mg
                              SolubilitySolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
                              Storage +15°C to +30°C
                              Do Not Freeze Ok to freeze
                              Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.
                              Toxicity Standard Handling
                              ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
                              Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
                              Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
                              Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
                              Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
                              Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
                              Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
                              Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
                              Jimenez, A., and Davies, J. 1980. Nature 287, 869.
                              Citation
                            • Ellen F. Cohn, et al. (2006) Abrupt Expression of TLR4 in TLR4-Deficient Macrophages Imposes a Selective Disadvantage: Genetic Evidence for TLR4-Dependent Responses to Endogenous, Nonmicrobial Stimuli. Journal of Immunology 176, 1185-1194.
                            • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
                            • Related Products & Applications

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                              Catalog Number Description  
                              345812 G 418 Sulfate, Sterile-Filtered Aqueous Solution, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Show Pricing & Availability

                              Categories

                              Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Other Inhibitors of Biological Interest > Protein Synthesis Inhibitors
                              Life Science Research > Inhibitors and Biochemicals > Biochemicals > Antibiotics > Antimicrobial Agents
                              Life Science Research > Cell Culture and Systems > Antibiotics & Selection Agents