344289 Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum

344289
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      Overview

      Replacement Information

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      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      344289-1SET
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          Glass bottle 1 set
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          Description
          OverviewContains 10 vials per set, each containing 10 ml of Freund's complete adjuvant homogenized in the following proportions by weight: 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier), and 0.1% Mycobacterium butyricum dry cells.
          Catalogue Number344289
          Brand Family Calbiochem®
          References
          Product Information
          CAS number9007-81-2
          FormClear amber to slightly cloudy liquid
          FormulationYellow, oily, bacterial suspension.
          Applications
          ApplicationFreund’s Complete Adjuvant, Modified, Mycobacterium butyricum, CAS 9007-81-2, provided as a set of 10 vials each containing: 85% Drakeol 5NF, 15% Arlacel A, and 0.1% M.butyricum dry cells.
          Application NotesImmunization
          Application CommentsComposition
          The Freund's Adjuvant set contains 10 identical ampules, each with 10 ml of Freund's Complete Adjuvant homogenized in the following preparations by weight:
          85% Drakeol 5 NF
          15% Arlacel A, mannide monooleate emulsifier
          0.1% Mycobacterium butyricum dry cells

          Sample Preparation
          1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.
          2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.
          3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
          4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSLS6860000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          344289

          Brochure

          Title
          Antibody Sourcebook!" Second Edition
          Keyhole Limpet Hemocyanin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-August-2007 RFH
          ApplicationImmunization
          Description1 Set (10 x 10 ml) each containing yellow, oily, bacterial suspension.
          BackgroundThe word "adjuvant" is derived from the Latin, adjuvare, meaning "to help." During immunization, an adjuvant is generally mixed with the antigen to enhance the rate and amount of antibody formed. The most popular complete adjuvant is that of Freund. It consists of killed and dried microbial cells suspended in an emulsifying oil. Adjuvant without the microbial cells is called "incomplete adjuvant." Complete adjuvant forms a stable water-in-oil emulsion with aqueous solution of antigen. When injected subcutaneously into animals, the emulsified antigen forms a depot from which antigen is delivered slowly and at sustained levels to the animal's antibody-synthesizing system. The induced antibody response is usually high and sustained. In guinea pigs, an additional benefit from adjuvant is the delayed appearance of hypersensitivity.
          FormClear amber to slightly cloudy liquid
          FormulationYellow, oily, bacterial suspension.
          CAS number9007-81-2
          RTECSLS6860000
          CommentsComposition
          The Freund's Adjuvant set contains 10 identical ampules, each with 10 ml of Freund's Complete Adjuvant homogenized in the following preparations by weight:
          85% Drakeol 5 NF
          15% Arlacel A, mannide monooleate emulsifier
          0.1% Mycobacterium butyricum dry cells

          Sample Preparation
          1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.
          2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.
          3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
          4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Toxicity Standard Handling