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S1120 | Fragile X CHEMI™ DNA Probe (digoxigenin-labeled)

10 assays  
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      Replacement Information
      Catalogue NumberS1120
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionFragile X CHEMI™ DNA Probe (digoxigenin-labeled)
      OverviewThe Fragile X Syndrome is identified by an ampiflication of the FMR1 (CGG)n repeat region. The Fragile X CHEMI™ probe is a digoxigenin-labeled DNA probe (pFxa1NHE) which can be used in conjunction with the Sure Blot® CHEMI™ Kit (Cat. No. S4250) to detect (CGG) repeats in the FMR-1 gene. The same results are obtained as when using the standard P32-labeled probes, but without radioactivity. The amplification of the FMR-1 gene is detected on Southern blots by an increase in the band sizes. Also provided is a ready-to-use mixture of unlabeled and digoxigenin-labeled Lambda DNA/Hind III Molecular Weight Markers for convenient visualization on both ethidium bromide-stained agarose gels and Southern blots.
      Product Information
      Detection methodChemiluminescence
      Application NotesFragile X CHEMI™ is intended for Research Use Only. For best results, target human genomic DNA should be double digested, to completion, with the restriction enzymes EcoR I and either Sac II, Nru I, or Eag I.

      S1120-1 Fragile X (pFxa1NHE) DNA Probe

      Locus: FMR1

      Volume: 250 μl Concentration : 10ng/μl; 25 μl per 10 ml of hybridization solution. Sufficient probe to make 100 ml of hybridization solution at a probe concentration of 25 ng/ml. (equal to processing 1500 cm2)

      Restriction Map: see insert.

      S1120-2 Lambda/Hind III Molecular Weight Markers

      Fragile X CHEMI™ Molecular Weight Markers are Lambda DNA cut with Hind III. Each vial contains a ready-to-use mixture of unlabeled and digoxigenin-labeled DNA fragments in gel loading solution. The unlabeled markers are included for convenient visualization on agarose gel after staining with ethidium bromide. The fragment sizes are: 23.1, 9.4, 6.6, 4.4, 2.3, 2.0, 1.0, 0.56, 0.125 kb. The 0.125 kb fragment may be difficult to detect, and will likely electrophorese off the end of the gel.

      Volume: 800 μl. Sufficient for 20 lanes, 10 gels with 2 lanes per gel, 40 μl per lane.

      Materials Required but not Provided

      20X PBS Tween®-20

      1M MgCl2 X-ray film/cassette(s)

      Sure Blot® CHEMI™ Hybridization & Detection Kit (Catalog Number S4250)

      DNA Extraction Kit (Catalog Number S4520)

      20X SSC Packets

      20% SDS

      Report Cover acetate sheets or plastic wrap

      Instructions for Use

      Molecular Weight Markers

      1. Thaw contents of S1120-2 at room temperature or 37°C and aliquot 40 μl per lane into a separate microcentrifuge tube.

      2. Incubate the microcentrifuge tube containing the aliquoted markers at 65°C for 3-5 minutes. Load 40 μl per lane onto an agarose gel and proceed with electrophoresis and transfer.

      Do not incubate the stock tube of markers at 65°C.

      Probe Hybridization

      For complete hybridization, detection, and stripping protocols please refer to the S4250 instruction manual.

      Procedural Cautions

      Temperature and buffer salt concentration (stringency) of hybridization and washing are important, as lower stringency can result in non-specific binding of the probe to other sequences, and higher stringency can result in lack of signal.

      Additional Comments

      1. The Fragile X CHEMI™ DNA Probe is optimized for chemiluminescent detection.

      2. Use 10 μg of restriction enzyme digested human DNA per lane.
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceCell Proliferation Assay: The biological activity of this lot of VEGF was determined by its mitogenic activity on human umbilical vein endothelial cells (HUVEC) with an ED50 of about 1.0 ng/mL. Results may vary depending on the cell line used. VEGF will also stimulate endothelial cells from other species.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended Storage

      The Fragile X CHEMI™ DNA Probe and Molecular Weight Markers should be stored at -15°C to -25°C.
      Packaging Information
      Material Size10 assays
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Fragile-X #8211; Digoxigenin - 2055321 2055321
      Fragile-X #8211; Digoxigenin - 2088999 2088999
      Fragile-X #8211; Digoxigenin - 2088999A 2088999A
      Fragile-X #8211; Digoxigenin - 2205966 2205966
      Fragile-X – Digoxigenin -2475767 2475767
      Fragile-X – Digoxigenin -2521417 2521417
      Fragile-X – Digoxigenin -2557690 2557690
      Fragile-X – Digoxigenin -2642549 2642549
      Fragile-X – Digoxigenin -2667342 2667342
      Fragile-X – Digoxigenin -2697612 2697612
      Fragile-X – Digoxigenin -2752616 2752616
      Fragile-X – Digoxigenin - 2370672 2370672
      Fragile-X – Digoxigenin - 2392282 2392282


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      Interplay between cyclin-dependent kinase 5 and glycogen synthase kinase 3 beta mediated by neuregulin signaling leads to differential effects on tau phosphorylation and amyloid precursor protein processing.
      Yi Wen,Emmanuel Planel,Mathieu Herman,Helen Y Figueroa,Lili Wang,Li Liu,Lit-Fui Lau,Wai Haung Yu,Karen E Duff
      The Journal of neuroscience : the official journal of the Society for Neuroscience 28 2008

      Show Abstract
      18322105 18322105
      Genotype-phenotype relationships in fragile X syndrome: a family study.
      Loesch, D Z, et al.
      Am. J. Hum. Genet., 53: 1064-73 (1993) 1993

      Show Abstract
      8213832 8213832
      Isolation of a human DNA sequence which spans the fragile X.
      Kremer, E J, et al.
      Am. J. Hum. Genet., 49: 656-61 (1991) 1991

      Show Abstract
      1882843 1882843
      Fragile X genotype characterized by an unstable region of DNA.
      Yu, S, et al.
      Science, 252: 1179-1181 (1991) 1991

      17797913 17797913
      Selection and characterization of recombinant clones that produce Mycobacterium leprae antigens recognized by antibodies in sera from household contacts of leprosy patients.
      R A Hartskeerl,R M van Rens,L F Stabel,M Y de Wit,P R Klatser
      Infection and immunity 58 1990

      Show Abstract Full Text Article
      1696931 1696931