FCCH025111 FlowCellect Oxidative Stress Characterization Kit

FCCH025111
25 Tests  
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      Overview

      Replacement Information
      Description
      Catalogue NumberFCCH025111
      Trade Name
      • FlowCellect
      DescriptionFlowCellect Oxidative Stress Characterization Kit
      OverviewOxidative stress has been found to play a key role in a number of pathological disorders. To aid researchers studying this phenomenon Millipore has developed the FlowCellect™ Oxidative Stress Characterization Kit. This kit contains the chemical and immunological reagents necessary to detect carbonyl groups introduced onto proteins by reactive oxygen species (ROS). The test method involves derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH). This chemical reaction results in proteins being covalently coupled to DNP at their carbonyl sites. The DNP-derivatized proteins are then incubated with a FITC conjugated monoclonal antibody that specifically binds to the DNP moiety. Subsequent detection with a bench-top Guava EasyCyte™ Plus flow cytometer allows quantification of the extent of oxidative stress within cells. Although the assay and all of the kit components were optimized using a Guava instrument, any flow cytometer can be utilized provided it contains a blue laser (488 nm) light source.
      Alternate Names
      • FlowCellect
      References
      Product Information
      Components
      • Anti-DNP -FITC
      • Derivatization Control Solution
      • 5X Assay Buffer
      • DNPH Solution
      • 10X Wash Buffer
      • User Manual
      Detection methodFluorescent
      Instrument compatibility
      • EasyCyte 8HT
      • EasyCyte Plus
      • EasyCyte Mini
      • EasyCyte
      Applications
      ApplicationOxidative stress has been found to play a key role in a number of pathological disorders.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon arrival the FITC conjugated anti-DNP antibody should be transferred to a -20°C freezer. The remaining kit components (FCCH025111-1) should be stored at 2 - 8ºC.
      All kit components are stable for up to six (6) months from date of receipt if stored and handled appropriately.
      Packaging Information
      Material Size25 Tests
      Transport Information
      Supplemental Information
      Specifications