FCCH100109 FlowCellect MitoStress Kit

FCCH100109
100 Tests  
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      Overview

      Replacement Information
      Description
      Catalogue NumberFCCH100109
      Trade Name
      • FlowCellect
      DescriptionFlowCellect MitoStress Kit
      OverviewMillipore’s FlowCellect™ MitoStress Kit includes MitoSOX Red, a live-cell permeant fluorogenic dye which targets the mitochondria and reacts with superoxide radicals and fluoresces yellow/red, and Annexin V conjugated to CF647 which binds to phosphatidylserine (PS) on the surface of apoptotic cells. The simultaneous use of these reagents allows researchers to obtain information on oxidative stress and apoptosis in one simple assay.

      MitoSOX Red is excitable by a 488nm laser and fluoresces maximally at 580 nm (Yellow fluorescence on the guava easyCyte 8HT). Annexin V is calcium-dependent phospholipid binding protein with high affinity for PS, a membrane component normally localized to the internal face of the cell membrane. It is conjugated to CF647 which is excited by a red laser and emits at 665 nm (Red2 fluorescence on the guava easyCyte 8HT). Control cells depict no Red2 fluorescence while apoptotic cells exhibit positive Red2 fluorescence. The kit can thus distinguish multiple populations: 1) Live cells with little or no superoxide 2) Stressed cells with oxidized MitoSOX Red (indicator of accumulated superoxide) but no Annexin V staining 3) Stressed and Early Apoptotic cells with oxidized MitoSOX Red and Annexin V binding and 4) Apoptotic Cells with Annexin V binding only., The MitoStress kit rapidly provides information on correlation of mitochondrial superoxide stress with apoptosis using minimal cellular sample. The entire assay can be performed in less than 30 minutes, once cellular samples are ready.
      Alternate Names
      • FlowCellect
      References
      Product Information
      Components
      • MitoSOX Red
      • Annexin V CF647
      • 10X Assay Buffer
      • User Manual
      Detection methodFluorescent
      Instrument compatibility
      • EasyCyte 8HT
      Applications
      ApplicationThis FlowCellect MitoStress Kit for Flow Cytometry includes MitoSOX Red, a live-cell permeant fluorogenic dye & Annexin V conjugated to CF647 which binds to phosphatidylserine (PS) on the surface of apoptotic cells.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size100 Tests
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Software Compatibility

      Catalog Number Description  
      0500-4120 InCyte Software for Guava® easyCyte HT Systems Show Pricing & Availability

      Product Families

      Related Products By: Brand Facete

      Categories

      Life Science Research > Cell Analysis > guava easyCyte Flow Cytometers > Flow Cytometry Kits & Reagents