FCCH025143 FlowCellect™ Cell Cycle Checkpoint ATM DNA Damage Kit

FCCH025143
25 Tests / Kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberFCCH025143
      Trade Name
      • FlowCellect
      DescriptionFlowCellect™ Cell Cycle Checkpoint ATM DNA Damage Kit
      OverviewMillipore’s FlowCellect™ DNA Damage (ATM) and Cell Cycle Analysis Kit is designed to identify the linkage between DNA damage in relation to cell cycle checkpoints in flow cytometry applications. The kit includes a directly conjugated Anti-phospho-ATM (Ser1981) Alexa Fluor® 488 conjugate plus a DNA dye (Propidium Iodide) which allows the researcher to perform bivariate analysis to identify where DNA damage takes place relative to the positioning within the cell cycle. The kit includes an optimized protocol with all of the necessary components to provide the researcher a true “plug and play” design for their research when studying DNA damage and cell cycle checkpoints. This immunocytochemical method is very sensitive, and it offers an assay to screen anti-tumor drugs at every phase of the cell cycle.

      In all, a comprehensive understanding of DNA damage and its association with cell cycle behavior can provide the researcher with useful information which will be important in interpreting the intrinsic nature of cell proliferation, apoptosis, DNA repair, and assist in the development of anti-neoplastic agents. Millipore’s FlowCellect™ DNA Damage (ATM) and Cell Cycle Analysis Kit is designed to allow the researcher to easily identify, discriminate, and quantitate cells undergoing DNA damage relative to its positioning within the cell cycle.

      All FlowCellect kits are optimized on guava® bench top flow cytometers. FlowCellect kits can be used on any flow cytometer following the same protocol providing researchers a reliable and fully validated solution to study DSBs and cell cycle distribution right in the comfort of their own lab. This kit contains optimized fixation, permeabilization, wash, and flow buffers to provide researchers with a complete solution for bivariate analysis to assess DNA damage in relation to positioning within the cell cycle.
      Alternate Names
      • Flow Cellect
      Background InformationAtaxia telangiectasia mutated (ATM) is a protein kinase that becomes activated in response to DNA damage, particularly when the damage involves formation of DNA double-strand breaks (DSBs). Detection of DNA damage due to DSBs is a fundamental method for assessing cell health and evaluation of anti-neoplastic agents. Bivariate analysis monitoring the extent of ATM phosphorylation along with a DNA dye offers a sensitive and valuable tool in studying the factors that induce DNA damage (measured by the level of DSBs) and/or affect DNA repair. Understanding where the first indication of DSBs take place during the cell cycle can help researchers understand the mechanisms involved in cell cycle arrest and DNA repair at critical cell cycle checkpoints. Malfunctions in the cell cycle in response to DNA damage can increase the incidence of cancer since the DNA repair machinery fails to operate properly. This is important because the cell has several systems for interrupting the cell cycle if the DNA repair machinery malfunctions. ATM has been shown to colocalize at sites of ionizing radiation–induced DNA damage and to phosphorylate the histone variant H2AX on Ser139. Evidence suggests that H2A.X phosphorylation may recruit checkpoint proteins to sites of DNA damage and play a role in DNA repair.

      Various factors can cause DNA double stranded breaks resulting in DNA damage. Physical factors such as UV and ionizing radiation, or chemical factors such as synthetic compounds (anti-tumor drugs) can all cause DNA damage. Regardless of the source to induce DNA damage, the end result will either cause cell cycle checkpoint arrest, apoptosis, or will trigger the DNA repair response to damage.
      References
      Product Information
      Components
      • 1. 20X Anti-p-ATM (Ser1981), clone 10H11.E12 - Alexa Fluor® 488 Conjugate: (Part No. CS206400) One vial containing 150 µL antibody.
      • 2. Propidium Iodide Solution: (Part No. CS204836) One vial containing 400 µL DNA dye.
      • 3. RNase A Reagent: (Part No. CS204821) One vial containing 25 µL.
      • 4. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL buffer.
      • 5. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL buffer.
      • 6. 5X Assay Buffer: (Part No. CS202124) One bottle containing 55 mL buffer.
      • 7. 1X Permeabilization Buffer: (Part No. CS203284) Two bottles containing 14 mL buffer.
      Detection methodFluorescent
      Applications
      ApplicationThis FlowCellect DNA Damage (ATM) & Cell Cycle Analysis Kit is designed to identify the linkage between DNA damage in relation to cell cycle checkpoints in flow cytometry applications.
      Key Applications
      • Flow Cytometry
      Biological Information
      Clone10H11.E12
      HostMouse
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceLot to lot in-process testing on all kit components
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      Storage and Shipping Information
      Storage Conditions4-8°C for antibodies and buffers
      Packaging Information
      Material Size25 Tests / Kit
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Software Compatibility

      Catalog Number Description  
      0500-4120 InCyte Software for Guava® easyCyte HT Systems Show Pricing & Availability

      Product Families

      Related Products By: Brand Facete

      Categories

      Life Science Research > Cell Analysis > guava easyCyte Flow Cytometers > Flow Cytometry Kits & Reagents