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FCCH100108 | FlowCellect Annexin Red Kit

100 Tests  
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      Replacement Information
      Catalogue NumberFCCH100108
      Trade Name
      • FlowCellect
      DescriptionFlowCellect Annexin Red Kit
      OverviewMillipore’s FlowCellect Annexin Red Kit provides a rapid and convenient assay to monitor early and late apotosis. The kit includes recombinant Annexin V conjugated to a red sensitive dye, CF647, to provide maximum sensitivity and 7-AAD, a cell impermeant dye which is an indicator of cell membrane structural integrity along with an assay buffer. 7-AAD is excluded from live, healthy cells and early apoptotic cells but stains late stage apoptotic and dead cells with red fluorescence by binding to nucleic acids in the cell. After staining cells with Flowcellect Annexin Red Kit, three populations of cells can be identified in this assay:

      • Non-apoptotic cells: Annexin V(-) and 7-AAD(-)
      • Early apoptotic cells: Annexin V(+) and 7-AAD(-)
      • Late stage apoptotic or dead cells: Annexin V(+) and 7-AAD(+)

      The kit permits users to pair the provided reagents with other reagents that fluoresce in the green and yellow channels of the flow cytometer. Sufficient reagents are provided for 100 tests. The kit includes all optimized fluorescently labeled reagents and buffers necessary for cell preparation and analysis.
      Alternate Names
      • FlowCellect
      Product Information
      • Annexin VCF647
      • 7AAD
      • 10X Assay Buffer
      • User Manual
      Detection methodFluorescent
      Instrument compatibility
      • EasyCyte 8HT
      ApplicationThis FlowCellect Annexin Red Kit for flow cytometry provides a rapid & convenient assay to monitor early & late apotosis.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size100 Tests
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      FlowCellect Annexin Red Kit - 13-0224 13-0224
      FlowCellect Annexin Red Kit - 13-0266 13-0266
      FlowCellect Annexin Red Kit - 13-0414 13-0414
      FlowCellect Annexin Red Kit - 14-0121 14-0121
      FlowCellect Annexin Red Kit - 14-0268 14-0268


      Reference overviewApplicationSpeciesPub Med ID
      Epigallocatechin-3-gallate up-regulates tumor suppressor gene expression via a reactive oxygen species-dependent down-regulation of UHRF1.
      Achour, Mayada, et al.
      Biochem. Biophys. Res. Commun., (2012) 2012

      Show Abstract
      Chromatin Immunoprecipitation (ChIP)Human23201574 23201574


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