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FCIM025118 | FlowCellect™ Human FOXP3 Treg Characterization Kit

25 Tests / Kit  
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      Replacement Information
      Catalogue NumberFCIM025118
      Trade Name
      • FlowCellect
      DescriptionFlowCellect™ Human FOXP3 Treg Characterization Kit
      OverviewWe have developed a multi-parameter flow cytometry assay for the characterization of human regulatory T-cells. Millipore’s FlowCellect™ Human FOXP3 Treg Characterization Kit includes three directly conjugated antibodies: CD3 PE/Cy5, CD4 FITC, and FOXP3 Alexa Fluor® 647, along with optimized fixation, permeabilization, wash, and assay buffers to provide researchers the ability to phenotypically distinguish human regulatory T-cells. Our combination of directly conjugated antibodies and optimized buffers allow for detection of CD3 and CD4 on the surface as well as FOXP3 within the nucleus simultaneously.

      Antibodies against CD3 and CD4 are provided in the kit to characterize cells by their surface marker expression. CD3 is a marker for the T-cell receptor, while CD4 is a glycoprotein that is found on T helper cells, monocytes, dendritic cells, macrophages, and regulatory T-cells . When looking at CD4 positive cells which are also positive for CD3 you are effectively eliminating the Monocytes, Macrophages, and dendritic cells present in peripheral blood or PBMC’s from your analysis. These cells which are CD3+, CD4+, and also FOXP3 + identify the regulatory T-cell population.
      Alternate Names
      • Flow Cellect
      Background InformationRegulatory T-cells (Tregs) are a subpopulation of helper T-cells which are CD3+, CD4+, CD25+, and express the transcription factor FOXP3. Characterization of these cells can be accomplished by performing multi-parameter flow cytometry to identify key combinations of the aforementioned surface and intracellular markers.

      Regulatory T-cells play a vital role in regulating the responses of the immune system. They suppress the activation of the immune system following infections, which helps maintain a balance or homeostasis. It has also been shown that some cancer patients have increased numbers of regulatory T-cells which may allow some malignant cells to escape the activation of the immune system due to the suppressive nature of the Tregs. Researchers are interested in the mechanism by which cancers are evading responses of the immune system as these could aid in the design of alternate treatment methods.

      A second and equally important function of regulatory T-cells is promoting tolerance to self-antigens. Regulatory T-cells act to ensure that self-antigens presented to the immune system do not illicit an immune response. This is yet another way that regulatory T-cells help to maintain homeostasis.

      Many researchers are interested in the area of self-tolerance as a way to identify what can be tolerated and what signals a response to antigens. In particular, it would be of great benefit to understand the immune response that is common in organ, tissue, and cellular transplantation from donors to recipients. Knowing how to block/suppress the response to foreign antigens would allow the transplanted organ/tissue/cells to pass as self and escape rejection by the immune system.
      Product Information
      • 1. 20X Anti-CD3-PE/Cy5, Antibody (Part No. CS206469) One vial containing 150 µL antibody.
      • 2. 20X Anti-CD4-FITC, Antibody (Part No. CS206470) One vial containing 150 µL antibody.
      • 3. 20X Anti-FOXP3-Alexa Fluor® 647, Antibody (Part No. CS206471) One vial containing 150 µL antibody.
      • 4. 4X Fixation/Permeabilization Buffer: (Part No. CS206472) One bottle containing 3.2 mL buffer.
      • 5. 1X Fixation/Permeabilization Diluent (Part No. CS206473) One bottle containing 9.5 mL buffer.
      • 6. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL buffer.
      • 7. 5X Assay Buffer: (Part No. CS202124) One bottle containing 55 mL buffer.
      • 8. 10X Permeabilization Buffer: (Part No. CS206474) One bottle containing 6.5 mL buffer.
      Detection methodFluorescent
      ApplicationThis Human FOXP3 Treg Characterization Kit for flow cytometry is a multi-parameter flow cytometry assay for the characterization of human regulatory T-cells.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceLot to lot in-process testing on all kit components
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions4-8°C for antibodies and buffers
      Packaging Information
      Material Size25 Tests / Kit
      Transport Information
      Supplemental Information

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