341635 | Fibronectin, Human Plasma

341635
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      341635-1MG
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          Plastic ampoule 1 mg
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          341635-5MG
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              Plastic ampoule 5 mg
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              Description
              OverviewNative fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
              Catalogue Number341635
              Brand Family Calbiochem®
              References
              ReferencesAkiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol. 59, 1.
              Krejci, K., and Fritz, H. 1976. FEBS Lett. 64, 152.


              Selected Citations
              Hoffmeister, M., et al. 2008. J. Biol. Chem. 283, 2297.


              Brown, S., et al. 2002. Nature 418, 200.


              Kim, M., FEBS Letters 581 1917.
              Product Information
              CAS number86088-83-7
              FormLiquid
              FormulationIn PBS, pH 7.3.
              Applications
              Biological Information
              Puritysingle major band by SDS-PAGE
              SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
              Concentration Label Please refer to vial label for lot-specific concentration
              Physicochemical Information
              Contaminantsnone detected by IEP with antiserum to fibronectin or normal human serum
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Standard Handling
              Storage -20°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
              Special InstructionsThaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              341635

              References

              Reference overview
              Akiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol. 59, 1.
              Krejci, K., and Fritz, H. 1976. FEBS Lett. 64, 152.


              Selected Citations
              Hoffmeister, M., et al. 2008. J. Biol. Chem. 283, 2297.


              Brown, S., et al. 2002. Nature 418, 200.


              Kim, M., FEBS Letters 581 1917.
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision01-July-2009 RFH
              DescriptionNative fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
              FormLiquid
              FormulationIn PBS, pH 7.3.
              Concentration Label Please refer to vial label for lot-specific concentration
              Recommended reaction conditions
              Protocol for Coating Tissue Culture Plates With Fibronectin Note: this protocol is meant to serve as a guideline. Optimization may be required for individual cell types.
              Note: This protocol is based on the use of 21 cm2 dishes (Corning 25010, Costar 3060, Falcon 3002, or an equivalent 60 mm dish) and 1 mg fibronectin; 1 mg fibronectin is sufficient for coating 10 dishes at 5 µg/cm2.
              1. Thaw the fibronectin by placing the vial in a 37°C water bath and leaving it undisturbed until completely thawed. DO NOT DISTURB THE VIAL AT ANY TIME DURING THE THAWING PROCESS! If the vial is disturbed or removed prior to complete thawing, the fibronectin will form a gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing. 2. After thawing, bring the solution to a final volume of 20 ml with sterile, serum-free culture medium that has been pre-warmed to 37°C. This yields a fibronectin solution of 50 µg/ml. 3. Add 2 ml of this solution to each of 10 tissue culture dishes and swirl gently to completely coat the growth surface. 4. Allow the dishes to remain at room temperature for ~45 min to permit binding of the fibronectin to the surface of the dish. 5. Tilt each dish and remove the fibronectin using a sterile pipet. Do not permit the pipet tip to touch or disturb the growth surface. 6. Add the cell suspension in medium directly to the fibronectin-coated dish.
              SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
              CAS number86088-83-7
              Puritysingle major band by SDS-PAGE
              Contaminantsnone detected by IEP with antiserum to fibronectin or normal human serum
              Storage -20°C
              Avoid freeze/thaw
              Do Not Freeze Ok to freeze
              Special InstructionsThaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).
              Toxicity Standard Handling
              Merck USA index14, 4075
              ReferencesAkiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol. 59, 1.
              Krejci, K., and Fritz, H. 1976. FEBS Lett. 64, 152.


              Selected Citations
              Hoffmeister, M., et al. 2008. J. Biol. Chem. 283, 2297.


              Brown, S., et al. 2002. Nature 418, 200.


              Kim, M., FEBS Letters 581 1917.