69036 Factor Xa, Restriction Grade, Bovine Plasma

69036
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      69036-2000UG
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          Glass bottle 2000 μg
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          69036-3
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              Plastic ampoule 100 μg
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              69036-500UG
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                  Glass bottle 500 μg
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                  Description
                  OverviewRestriction Grade Factor Xa is a highly purified preparation isolated from bovine plasma and activated with Russell's viper venom. This preparation is purified to near homogeneity and shows no secondary cleavage from contaminating proteases. The preparation is also functionally tested for activity with fusion proteins. Like enterokinase, Factor Xa cleaves at the C-terminal side of the recognition sequence (IleGluGlyArg↓) and can therefore be used for removing all vector-encoded sequences from appropriately designed constructs.

                  Specific Activity: ≥150 IU/mg

                  One IU is defined as the amount of enzyme that will release 1 µmole of p-nitroaniline per minute using methanesulfonyl-D-leucyl-glycyl-arginyl-paranitroanilide at 37°C, pH 8.4.

                  One µg of enzyme cleaves 50 µg Cleavage Control Protein to >95% completion in 16 hr at 25°C in a buffer containing 50 mM Tris-HCl pH 8.0, 100 mM NaCl, and 5 mM CaCl2.
                  Catalogue Number69036
                  Brand Family Novagen®
                  References
                  Product Information
                  100 µgRestriction Grade Factor Xa
                  2 mlFactor Xa Dilution/Storage Buffer
                  1 ml10X Factor Xa Cleavage Buffer
                  10 µgCleavage Control Protein
                  Applications
                  Biological Information
                  Biological activityOne microgram of enzyme cleaves 50 µg Xa Cleavage Control Protein to >95% completion in 16 hours at 25°C in a buffer containing 50 mM Tris-HCl pH 8.0, 100 mM NaCl, and 5 mM CaCl₂.
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Blue Ice Only
                  Toxicity Multiple Toxicity Values, refer to MSDS
                  Storage -20°C
                  Avoid freeze/thaw Avoid freeze/thaw
                  Do not freeze Ok to freeze
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Certificates of Analysis

                  TitleLot Number
                  69036

                  Brochure

                  Title
                  Bulk Product Guide
                  Bulk packaging
                  Protein Purification and Detection Tools
                  Protein Purification and Preparation

                  Citations

                  Title
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                • Hongjun Jin, et al. (2007) The human CC chemokine MIP-1 dimer is not competent to bind to the CCR5 receptor. Journal of Biological Chemistry 282, 27976-27983.
                • Nadine Kröning, et al. (2007) ATP binding to the KTN/RCK subunit KtrA from the K+-uptake system KtrAB of Vibrio alginolyticus: Its role in the formation of the KtrAB complex and its requirement in vivo. Journal of Biological Chemistry 282, 14018-14027.
                • Saumen Pal, et al. (2006) An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis. Journal of Cell Biology 174, 1047-1058.
                • Chih-Ying Chen and Frances M. Brodsky. (2005) Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo. Journal of Biological Chemistry 280, 6109-6117.
                • Izhack Cherny, Liat Rockah and Ehud Gazit. (2005) The YoeB toxin is a folded protein that forms a physical complex with the unfolded YefM antitoxin: Implications for a structural-based differential stability of toxin-antitoxin systems. Journal of Biological Chemistry 280, 30063-30072.
                • Masanobu Chinami, et al. (2005) Binding of HTm4 to cyclin-dependent kinase (Cdk)-associated phosphatase (KAP)·Cdk2·cyclin A complex enhances the phosphatase activity of KAP, dissociates cyclin A, and facilitates KAP dephosphorylation of Cdk2. Journal of Biological Chemistry 280, 17235-17242.
                • Gianguido Coffa, et al. (2005) On the relationships of substrate orientation, hydrogen abstraction, and product stereochemistry in single and double dioxygenations by soybean lipoxygenase-1 and its Ala542Gly mutant. Journal of Biological Chemistry 280, 38756-38766.
                • Rey-Ting Guo, et al. (2005) Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis. Journal of Biological Chemistry 280, 20762-20774.
                • Min-Feng Hsu, et al. (2005) Understanding the maturation process and inhibitor design of SARS-CoV 3CLpro from the crystal structure of C145A in a product-bound form. Journal of Biological Chemistry 280, 31257-31266.
                • Margarita L. Malakhova, et al. (2005) Point mutational analysis of the liganding site in human glycolipid transfer protein: functionally of the complex. Journal of Biological Chemistry 280, 26312-26320.
                • Koji Ohira, et al. (2005) A truncated tropo-myosine-related kinase B receptor, T1, regulates glial cell morphology via rho GDP dissociation inhibitor 1. Journal of Neuroscience 25, 1343-1353.
                • Ken Okada and Toshiharu Hase. (2005) Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1. Journal of Biological Chemistry 280, 60672-20679.
                • S. Dean Rider, et al. (2005) The protozoan parasite Cryptosporidium parvum possesses two functionally and evolutionarily divergent replication protein A large subunits. Journal of Biological Chemistry 280, 31460-31469.
                • Andreas Savelsbergh, et al. (2005) Control of phosphate release from elongation factor G by ribosomal protein L7/12. European Molecular Biology Organization Journal 24, 4316-4323.
                • Irene Soderhall, et al. (2005) An ancient role for a prokineticin domain in invertebrate hematopoiesis. journal of Immunology 174, 6153-6160.
                • Wei Zhang, John S. Olson and George N. Phillips, Jr.. (2005) Biophysical and kinetic characterization of HemAT, an aerotaxis receptor from Bacillus subtilis. Biophysical Journal 88, 2801-2814.
                • Domenico Sanfelice, et al. (2004) Letter to the editor: resonance assignment and secondary structire of the La motif. Journal of Biomolecular NMR 29, 449-450.
                • Thomas Lauber, et al. (2001) Accurate disulfide formation in Escherichia coli: overexpression and characterization of the first domain (HF6478) of the multiple Kazal-type inhibitor LEKTI. Protein Expression and Purification 22, 108-112.
                • Jennifer S. Laurence, et al. (2000) CC Chemokine MIP-1 can function as a monomer and depends on Phe13 for receptor binding. Biochemistry 39, 3401-3409.
                • User Protocols

                  Title
                  TB205 Factor Xa Kits Rev. G 0413JN