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344146 | FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli

344146
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      344146-100UG
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      Stocked 
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      Available
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          Glass bottle 100 μg
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          Description
          OverviewRecombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
          Catalogue Number344146
          Brand Family Calbiochem®
          SynonymsFTase, Farnesyl Proteintransferase
          References
          ReferencesZimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
          Reiss, Y., et al. 1990. Cell 62, 81.
          Product Information
          FormLiquid
          FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
          Applications
          ApplicationFPTase, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, is composed of an74 kDa α- and 47 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence.
          Biological Information
          Biological activity1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
          Purity≥90% by SDS-PAGE
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          344146

          References

          Reference overview
          Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
          Reiss, Y., et al. 1990. Cell 62, 81.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision26-June-2008 RFH
          SynonymsFTase, Farnesyl Proteintransferase
          DescriptionRecombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion protein with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
          FormLiquid
          FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
          Purity≥90% by SDS-PAGE
          Biological activity1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesZimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
          Reiss, Y., et al. 1990. Cell 62, 81.