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21-169 | FOPflash (mutant TCF binding sites)

5 µg  
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      Replacement Information
      Catalogue Number21-169
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionFOPflash (mutant TCF binding sites)
      Product Information
      ApplicationTransfection grade T cell factor (TCF) reporter plasmid containing 2 full & one incomplete copy of the Tcf binding site (mutated) followed by 3 copies in the reverse orientation. Serves as a negative control to TOPflash.
      Key Applications
      • Transfection
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.
      Gene Symbol
      • TCF1
      • MODY3
      • HNF1
      • HNF-1A
      • TCF-1
      • HNF1A
      • LFB1
      • HNF1a
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P20823 # Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
      SIZE: 631 amino acids; 67356 Da
      SUBUNIT: Binds DNA as a dimer.
      DISEASE: SwissProt: P20823 # Defects in HNF1A may predispose to hepatic adenomas [MIM:142330]. Hepatic adenomas are benign tumors at risk of malignant transformation. Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. & Defects in HNF1A are the cause of maturity onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY [MIM:606391] is a form of diabetes characterized by an autosomal dominant mode of inheritance, age of onset of 25 years or younger and a primary defect in insulin secretion. The clinical phenotype of MODY3 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications. & Defects in HNF1A are a cause of susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100].
      SIMILARITY: SwissProt: P20823 ## Belongs to the HNF1 homeobox family. & Contains 1 homeobox DNA-binding domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size5 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      FOPflash (TCF Reporter Plasmid) (TK-luciferase reporter) - 2444125 2444125
      FOPflash (TCF Reporter Plasmid) (TK-luciferase reporter) - 1969879 1969879
      FOPflash (TCF Reporter Plasmid) (TK-luciferase reporter) - 2168378 2168378
      FOPflash (TCF Reporter Plasmid) (TK-luciferase reporter) - 2197530 2197530
      FOPflash (TCF Reporter Plasmid) (TK-luciferase reporter) 5.5kb - 1982671 1982671
      FOPflash (TCF Reporter Plasmid) (TK-luciferase reporter)5.5kb - 2506503 2506503
      FOPflash (TCF Reporter Plasmid) - DAM1636020 DAM1636020
      FOPflash (TCF Reporter Plasmid) - DAM1682605 DAM1682605
      FOPflash (TCF Reporter Plasmid) - DAM1770434 DAM1770434
      FOPflash (TCF Reporter Plasmid) - NG1904283 NG1904283
      FOPflash (TCF Reporter Plasmid) -2726761 2726761
      FOPflash (TCF Reporter Plasmid) -2752630 2752630
      FOPflash (mutant TCF binding sites) - 0612047636 0612047636
      FOPflash (mutant TCF binding sites) - 0702052445 0702052445
      FOPflash (mutant TCF binding sites) - 18909 18909
      FOPflash (mutant TCF binding sites) - 19494 19494
      FOPflash (mutant TCF binding sites) - 19494a 19494a
      FOPflash (mutant TCF binding sites) - 30860 30860


      Reference overviewPub Med ID
      Interleukin-18/WNT1-inducible signaling pathway protein-1 signaling mediates human saphenous vein smooth muscle cell proliferation.
      Venkatapuram Seenu Reddy,Anthony J Valente,Patrice Delafontaine,Bysani Chandrasekar
      Journal of cellular physiology 226 2011

      Show Abstract
      21321938 21321938
      Stabilization of beta-catenin by a Wnt-independent mechanism regulates cardiomyocyte growth.
      Haq, Syed, et al.
      Proc. Natl. Acad. Sci. U.S.A., 100: 4610-5 (2003) 2003

      Show Abstract
      12668767 12668767
      Selective small molecule inhibitors of glycogen synthase kinase-3 modulate glycogen metabolism and gene transcription
      Coghlan, M P, et al
      Chem Biol, 7:793-803 (2000) 2000

      11033082 11033082
      The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF.
      Ishitani, T, et al.
      Nature, 399: 798-802 (1999) 1999

      Show Abstract
      10391247 10391247
      Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse.
      Korinek, V, et al.
      Mol. Cell. Biol., 18: 1248-56 (1998) 1998

      Show Abstract
      9488439 9488439
      The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors.
      Roose, J, et al.
      Nature, 395: 608-12 (1998) 1998

      Show Abstract
      9783587 9783587
      Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF
      van de Wetering, M, et al
      Cell, 88:789-99 (1997) 1997

      9118222 9118222