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14-343 | FKHR Protein, 100 µg

100 µg  
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      Replacement Information
      Catalogue Number14-343
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionFKHR Protein, 100 µg
      OverviewN-terminal, Glutathione-Stransferase (GST) tagged, full-length fusion protein corresponding to human FKHR
      Product Information
      ApplicationN-terminal, Glutathione-Stransferase (GST) tagged, full-length fusion protein corresponding to human FKHR.
      Key Applications
      • Kinase Assay
      • Western Blotting
      Biological Information
      SourceRecombinant human expressed in E. coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThis gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.
      Gene Symbol
      • FOXO1
      • FKHR
      • FKH1
      • FOXO1A
      Purification MethodGlutathione-agarose
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q12778 # Transcription factor.
      SIZE: 655 amino acids; 69662 Da
      SUBUNIT: Interacts with LRPPRC (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus.
      TISSUE SPECIFICITY: Ubiquitous.
      PTM: Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Thr-24, Ser-256 and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, which leads to nuclear exclusion and loss of function. Phosphorylation of Ser- 329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.
      DISEASE: SwissProt: Q12778 # Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.
      SIMILARITY: SwissProt: Q12778 ## Contains 1 fork-head DNA-binding domain.
      Molecular Weight105kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation by Akt1
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -70°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      FKHR (recombinant protein expressed in E. coli) - 2444166 2444166
      FKHR - 20295 20295
      FKHR - 24183 24183
      FKHR - 26661 26661
      FKHR - 28896 28896
      FKHR - DAM1557570 DAM1557570
      FKHR - DAM1780412 DAM1780412


      When using p81 paper (phosphocellulose squares), what is the purpose of the acetone wash? The purpose of washing the p81 squares in acetone is mostly to help them dry faster, but it also can help to eliminate low specificity binding.