325902 | FKBP12, GST-Fusion, Human, Recombinant, E. coli

325902
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      325902-60UG
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          Plastic ampoule 60 μg
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          Description
          OverviewFull-length recombinant, human FK506 binding protein 12-rapamycin associated protein 1 (FKBP12) (accession number = NP000792) fused to GST at the N-terminus and expressed in E.coli. FKBP12 is a cytosolic receptor for both rapamycin and FK506 that also serves as a natural cofactor for rapamycin's inhibition of mTOR. The molecular weight of 38,000 Da includes the GST moiety.
          One vial is enough for inhibitor screening of four K-LISA™ mTOR Activity Kits (CBA055) (400 wells).Upon initial thawing, aliquot and store at -70° C.
          Catalogue Number325902
          Brand Family Calbiochem®
          SynonymsFK506 binding protein 1A, 12 kDa, FKBP1A
          Application Data
          The activity of mTOR was assessed without inhibitor and in the presence of rapamycin alone (Cat. No. 553210), FKBP12 alone (Cat. No. 325902), FKBP12/rapamycin complex, and Wortmannin (Cat. No. 681675), using the K-LISA™ mTOR Activity Kit (Cat. No. CBA055) as outlined in the Detailed Protocol. The FKBP12/rapamaycin inhibitor complex was pre-assembled by mixing 62.5 µg/ml FKBP12 and 50 µM rapamycin on ice for 10 min. The mTOR Standard (50 µl) was incubated for 15 min on ice with FKBP12 (2 µl: final concentration 2.5 µg/ml), rapamycin (2 µl; final concentration 2 µM). FKPP12/rapamycin complex (final concentration 2.5 µg/ml, 2 µM rapamycin) or wortmannin (final concentration 10 µM). The kinase reaction was initiated by transferring each mixture to a corresponding well containing 2X kinase buffer and ATP. The results were assessed as outlined in the user protocol.
          References
          ReferencesJin, Y.J., et al. 1991. Proc Natl Acad Sci USA 88, 6677.
          Product Information
          FormLiquid
          FormulationIn PBS, 10% glycerol, pH 7.2.
          Applications
          ApplicationFKBP12, GST-Fusion, Human, Recombinant, E. coli is a full-length protein that is fused to GST at the N-terminus and expressed in E.coli. FKBP12 is a cytosolic receptor for both rapamycin and FK506.
          Biological Information
          Purity≥90% by SDS-PAGE
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          325902

          References

          Reference overview
          Jin, Y.J., et al. 1991. Proc Natl Acad Sci USA 88, 6677.

          Brochure

          Title
          Biologics 33.2
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-June-2007 JSW
          SynonymsFK506 binding protein 1A, 12 kDa, FKBP1A
          Application Data
          The activity of mTOR was assessed without inhibitor and in the presence of rapamycin alone (Cat. No. 553210), FKBP12 alone (Cat. No. 325902), FKBP12/rapamycin complex, and Wortmannin (Cat. No. 681675), using the K-LISA™ mTOR Activity Kit (Cat. No. CBA055) as outlined in the Detailed Protocol. The FKBP12/rapamaycin inhibitor complex was pre-assembled by mixing 62.5 µg/ml FKBP12 and 50 µM rapamycin on ice for 10 min. The mTOR Standard (50 µl) was incubated for 15 min on ice with FKBP12 (2 µl: final concentration 2.5 µg/ml), rapamycin (2 µl; final concentration 2 µM). FKPP12/rapamycin complex (final concentration 2.5 µg/ml, 2 µM rapamycin) or wortmannin (final concentration 10 µM). The kinase reaction was initiated by transferring each mixture to a corresponding well containing 2X kinase buffer and ATP. The results were assessed as outlined in the user protocol.
          DescriptionFull-length recombinant, human FK506 binding protein 12-rapamycin associated protein 1 (FKBP12) (accession number = NP000792) fused to GST at the N-terminus and expressed in E.coli. FKBP12 is a cytosolic receptor for both rapamycin and FK506 that also serves as a natural cofactor for rapamycin's inhibition of mTOR. The molecular weight of 38,000 Da includes the GST moiety.
          FormLiquid
          FormulationIn PBS, 10% glycerol, pH 7.2.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity≥90% by SDS-PAGE
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesJin, Y.J., et al. 1991. Proc Natl Acad Sci USA 88, 6677.